Thirteen polymorphic microsatellite DNA loci from whiptails of the genus Aspidoscelis (Teiidae: Squamata) and related cnemidophorine lizards

被引：4
|
作者：
Crawford, Nicholas G. [1 ,3 ]
Zaldivar-Rae, Jaime [2 ]
Hagen, Cris [3 ]
Schable, Amanda [3 ]
Rosenblum, Erica Bree [4 ,5 ]
Graves, Jeff A. [6 ]
Reeder, Tod W. [1 ]
Ritchie, Michael G. [6 ]
Glenn, Travis C. [3 ]
机构：
[1] San Diego State Univ, Dept Biol, San Diego, CA 92182 USA
[2] Univ Nacl Autonoma Mexico, Inst Ecol, Lab Conducta Anim, Mexico City 04510, DF, Mexico
[3] Univ Georgia, Savannah River Ecol Lab, Aiken, SC 29802 USA
[4] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
[5] Lawrence Berkely Natl Lab, Dept Genome Sci, Berkeley, CA 94720 USA
[6] Univ St Andrews, St Andrews KY16 9TS, Fife, Scotland
来源：
MOLECULAR ECOLOGY RESOURCES | 2008年 / 8卷 / 01期
关键词：
Ameiva; Aspidoscelis; Cnemidophorus; lizard; microsatellite; PCR primers; SSR; STR; teiid; whiptail;
D O I：
10.1111/j.1471-8286.2007.01930.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We describe polymerase chain reaction primers and amplification conditions for 13 microsatellite DNA loci isolated from two bisexual species of whiptail lizards Aspidoscelis costata huico and Aspidoscelis inornata. Primers were tested on either 16 or 48 individuals of A. c. huico and/or 26 individuals of A. inornata. Ten of the 13 primers were also tested against a panel of 31 additional whiptail taxa. We detected three to nine alleles per locus in A. c. huico and four to 19 alleles per locus in A. inornata, with observed heterozygosity ranging from 0.60 to 0.87 and from 0.15 to 1.00, respectively. These primers will be an important resource for surveys of genetic variation in these lizards.
引用
收藏
页码：219 / 223
页数：5
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