pancreatic duct adenocarcinoma;
DNA methylation;
E-cadherin;
hamster;
nitrosamine;
D O I:
10.1016/j.bbrc.2005.08.037
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
The expression of the E-cadherin gene and its DNA methylation status in the 5' upstream region were investigated in pancreatic duct adenocarcinomas (PDAs) induced by N-nitrosobis(2-oxopropyl)amine (BOP) in hamsters. Female Syrian golden hamsters received 70 mg/kg BOP, followed by repeated exposure to an augmentation pressure regimen consisting of a choline-deficient diet combined with DL-ethionine then L-methionine and a further administration of 20 mg/kg BOP. A total of 15 PDAs were obtained, along with total RNA for assessment of expression by real-time quantitative reverse transcription-polymerase chain reaction. The expression of the E-cadherin was significantly reduced in PDAs (p < 0.05) compared with normal pancreatic tissue. For the analysis of methylation status, bisulfite sequencing was performed with two normal pancreatic tissues and six tumors. The normal pancreatic tissue was all demethylated in this region of E-cadherin. In contrast, six PDAs were highly methylated, correlating with reduced expression of the E-cadherin. These results suggest that aberrant DNA methylation of the E-cadherin gene may play a role in the development of PDAs induced by BOP in hamsters. (c) 2005 Elsevier Inc. All rights reserved.
机构:
Univ Toronto, Toronto Med Labs, Univ Hlth Network, Dept Pathol, Toronto, ON, CanadaUniv Toronto, Toronto Med Labs, Univ Hlth Network, Dept Pathol, Toronto, ON, Canada
Chetty, Runjan
Serra, Stefano
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Univ Toronto, Toronto Med Labs, Univ Hlth Network, Dept Pathol, Toronto, ON, CanadaUniv Toronto, Toronto Med Labs, Univ Hlth Network, Dept Pathol, Toronto, ON, Canada
Serra, Stefano
Asa, Sylvia L.
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Univ Toronto, Toronto Med Labs, Univ Hlth Network, Dept Pathol, Toronto, ON, CanadaUniv Toronto, Toronto Med Labs, Univ Hlth Network, Dept Pathol, Toronto, ON, Canada
机构:
Kagoshima Univ, Grad Sch Med Sci, Dept Digest Surg Breast & Thyroid Surg, 8-35-1 Sakuragaoka, Kagoshima 8908520, JapanKagoshima Univ, Grad Sch Med Sci, Dept Digest Surg Breast & Thyroid Surg, 8-35-1 Sakuragaoka, Kagoshima 8908520, Japan
Yonemori, Keiichi
Kurahara, Hiroshi
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Kagoshima Univ, Grad Sch Med Sci, Dept Digest Surg Breast & Thyroid Surg, 8-35-1 Sakuragaoka, Kagoshima 8908520, JapanKagoshima Univ, Grad Sch Med Sci, Dept Digest Surg Breast & Thyroid Surg, 8-35-1 Sakuragaoka, Kagoshima 8908520, Japan
Kurahara, Hiroshi
Maemura, Kosei
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Kagoshima Univ, Grad Sch Med Sci, Dept Digest Surg Breast & Thyroid Surg, 8-35-1 Sakuragaoka, Kagoshima 8908520, JapanKagoshima Univ, Grad Sch Med Sci, Dept Digest Surg Breast & Thyroid Surg, 8-35-1 Sakuragaoka, Kagoshima 8908520, Japan
Maemura, Kosei
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Mataki, Yuko
Sakoda, Masahiko
论文数: 0引用数: 0
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Kagoshima Univ, Grad Sch Med Sci, Dept Digest Surg Breast & Thyroid Surg, 8-35-1 Sakuragaoka, Kagoshima 8908520, JapanKagoshima Univ, Grad Sch Med Sci, Dept Digest Surg Breast & Thyroid Surg, 8-35-1 Sakuragaoka, Kagoshima 8908520, Japan
Sakoda, Masahiko
Iino, Satoshi
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Kagoshima Univ, Grad Sch Med Sci, Dept Digest Surg Breast & Thyroid Surg, 8-35-1 Sakuragaoka, Kagoshima 8908520, JapanKagoshima Univ, Grad Sch Med Sci, Dept Digest Surg Breast & Thyroid Surg, 8-35-1 Sakuragaoka, Kagoshima 8908520, Japan
Iino, Satoshi
Ueno, Shinichi
论文数: 0引用数: 0
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机构:
Kagoshima Univ, Grad Sch Med Sci, Dept Clin Oncol, Kagoshima 8908520, JapanKagoshima Univ, Grad Sch Med Sci, Dept Digest Surg Breast & Thyroid Surg, 8-35-1 Sakuragaoka, Kagoshima 8908520, Japan
Ueno, Shinichi
Shinchi, Hiroyuki
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Kagoshima Univ, Grad Sch Med Sci, Dept Hlth Sci, Kagoshima 8908520, JapanKagoshima Univ, Grad Sch Med Sci, Dept Digest Surg Breast & Thyroid Surg, 8-35-1 Sakuragaoka, Kagoshima 8908520, Japan
Shinchi, Hiroyuki
Natsugoe, Shoji
论文数: 0引用数: 0
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Kagoshima Univ, Grad Sch Med Sci, Dept Digest Surg Breast & Thyroid Surg, 8-35-1 Sakuragaoka, Kagoshima 8908520, JapanKagoshima Univ, Grad Sch Med Sci, Dept Digest Surg Breast & Thyroid Surg, 8-35-1 Sakuragaoka, Kagoshima 8908520, Japan
机构:
Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Ribeirao Preto, SP, Brazil
Univ Fed Piaui, Parnaiba, PI, BrazilUniv Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Ribeirao Preto, SP, Brazil
Motta, F. J. N.
Valera, E. T.
论文数: 0引用数: 0
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机构:
Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Puericultura & Pediatria, Ribeirao Preto, SP, BrazilUniv Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Ribeirao Preto, SP, Brazil
Valera, E. T.
Lucio-Eterovic, A. K. B.
论文数: 0引用数: 0
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机构:
Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Puericultura & Pediatria, Ribeirao Preto, SP, BrazilUniv Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Ribeirao Preto, SP, Brazil
Lucio-Eterovic, A. K. B.
Queiroz, R. G. P.
论文数: 0引用数: 0
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机构:
Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Puericultura & Pediatria, Ribeirao Preto, SP, BrazilUniv Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Ribeirao Preto, SP, Brazil
Queiroz, R. G. P.
Neder, L.
论文数: 0引用数: 0
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机构:
Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Patol, Ribeirao Preto, SP, BrazilUniv Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Ribeirao Preto, SP, Brazil
Neder, L.
Scrideli, C. A.
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Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Puericultura & Pediatria, Ribeirao Preto, SP, BrazilUniv Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Ribeirao Preto, SP, Brazil
Scrideli, C. A.
Machado, H. R.
论文数: 0引用数: 0
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机构:
Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Anat & Cirurgia, Ribeirao Preto, SP, BrazilUniv Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Ribeirao Preto, SP, Brazil
Machado, H. R.
Carlotti-Junior, C. G.
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Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Anat & Cirurgia, Ribeirao Preto, SP, BrazilUniv Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Ribeirao Preto, SP, Brazil
Carlotti-Junior, C. G.
Marie, S. K. N.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Sao Paulo, Fac Med, Dept Neurol, Sao Paulo, BrazilUniv Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Ribeirao Preto, SP, Brazil
Marie, S. K. N.
Tone, L. G.
论文数: 0引用数: 0
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机构:
Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Puericultura & Pediatria, Ribeirao Preto, SP, BrazilUniv Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Ribeirao Preto, SP, Brazil
机构:
Hebei Med Univ, Dept Mol Biol, Shijiazhuang, Peoples R ChinaHebei Med Univ, Dept Mol Biol, Shijiazhuang, Peoples R China
Li, Yan
Ran, Ran
论文数: 0引用数: 0
h-index: 0
机构:
Hebei Med Univ, Hosp 4, Dept Gynecol, Jiankang St 12, Shijiazhuang 050011, Peoples R ChinaHebei Med Univ, Dept Mol Biol, Shijiazhuang, Peoples R China
Ran, Ran
Guan, Yingxia
论文数: 0引用数: 0
h-index: 0
机构:
Hebei Med Univ, Hosp 4, Dept Gynecol, Jiankang St 12, Shijiazhuang 050011, Peoples R ChinaHebei Med Univ, Dept Mol Biol, Shijiazhuang, Peoples R China
Guan, Yingxia
Zhu, Xiaoxiong
论文数: 0引用数: 0
h-index: 0
机构:
Hebei Med Univ, Hosp 4, Dept Gynecol, Jiankang St 12, Shijiazhuang 050011, Peoples R ChinaHebei Med Univ, Dept Mol Biol, Shijiazhuang, Peoples R China
Zhu, Xiaoxiong
Kang, Shan
论文数: 0引用数: 0
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机构:
Hebei Med Univ, Hosp 4, Dept Gynecol, Jiankang St 12, Shijiazhuang 050011, Peoples R ChinaHebei Med Univ, Dept Mol Biol, Shijiazhuang, Peoples R China