Expression of Plasmodium falciparum 3D7 STEVOR proteins for evaluation of antibody responses following malaria infections in naive infants

被引：19

作者：

Schreiber, N. ^{[2
]}

Khattab, A. ^{[1
]}

Petter, M. ^{[1
]}

Marks, F. ^{[2
,3
]}

Adjei, S. ^{[4
]}

Kobbe, R. ^{[2
]}

May, J. ^{[2
]}

Klinkert, M. -Q. ^{[1
]}

机构：

[1] Bernhard Nocht Inst Trop Med, Dept Mol Med, D-20359 Hamburg, Germany

[2] Bernhard Nocht Inst Trop Med, Infect Dis Epidemiol Grp, D-20359 Hamburg, Germany

[3] Int Vaccine Inst, Seoul 151600, South Korea

[4] KNUST Univ PO, Kumasi Ctr Collaborat Res Trop Med, Kumasi, Ghana

来源：

PARASITOLOGY | 2008年 / 135卷 / 02期

关键词：

recombinant STEVOR; Plasmodium falciparum; immune responses; infants;

D O I：

10.1017/S0031182007003794

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Clinical immunity to Plasmodium falciparum malaria develops after repeated exposure to the parasite. At least 2 P. falciparum variant antigens encoded by multicopy gene families (var and rif) are targets of this adaptive antibody - mediated immunity. A third multigene family of variant antigens comprises the stevor genes. Here, 4 different stevor sequences were selected for cloning and expression in Escherichia coli and His(6)-tagged fusion proteins were used for assessing the development of immunity. In a cross-sectional analysis of clinically immune adults living in a malaria endemic area in Ghana, high levels of anti-STEVOR IgG antibody titres were determined in ELISA. A cross-sectional study of 90 nine-month-old Ghanaian infants using I recombinant STEVOR showed that the antibody responses correlated positively with the number of parasitaemia episodes. In a longitudinal investigation of 17 immunologically naive 9-month-old infants, 3 different patterns of anti-STEVOR antibody responses could be distinguished (high, transient and low). Children with high anti-STEVOR-antibody levels exhibited an elevated risk for developing parasitaemia episodes. Overall, a protective effect could not be attributed to antibodies against the STEVOR proteins chosen for the study presented here.

引用

页码：155 / 167

页数：13

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