Photodynamic Effects of Pterin on HeLa Cells

被引：20

作者：

Denofrio, M. Paula ^{[2
]}

Lorente, Carolina ^{[2
]}

Breitenbach, Thomas ^{[3
]}

Hatz, Sonja ^{[3
]}

Cabrerizo, Franco M. ^{[1
]}

Thomas, Andres H. ^{[2
]}

Ogilby, Peter R. ^{[3
]}

机构：

[1] Univ Nacl San Martin, Inst Invest Biotecnol, Inst Tecnol Chascomus IIB INTECH, CONICET, Camino Circunv, Chascomus, Argentina

[2] Univ Nacl La Plata, Dept Quim, Fac Ciencias Exactas, Inst Invest Fisicoquim Teor & Aplicadas INIFTA, La Plata, Buenos Aires, Argentina

[3] Univ Aarhus, Dept Chem, Ctr Oxygen Microscopy & Imaging, Aarhus, Denmark

来源：

PHOTOCHEMISTRY AND PHOTOBIOLOGY | 2011年 / 87卷 / 04期

基金：

新加坡国家研究基金会;

关键词：

SINGLET OXYGEN; 2'-DEOXYGUANOSINE 5'-MONOPHOSPHATE; FOLIC-ACID; DNA; TETRAHYDROBIOPTERIN; PHOTOSENSITIZATION; BIOSYNTHESIS; DERIVATIVES; APOPTOSIS; LIFETIME;

D O I：

10.1111/j.1751-1097.2011.00922.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pterins, heterocyclic compounds widespread in biological systems, participate in relevant biological processes and are able to act as photosensitizers. In the present study, we ascertained that 2-aminopteridin-4(3H)-one, abbreviated as Ptr, is readily incorporated into and/or onto cervical cancer cells (HeLa) and that these cells die upon UV-A irradiation of Ptr. Cell death was assessed using two tests: (1) the Rhodamine 123 fluorescence assay for mitochondrial viability and (2) the Trypan Blue assay for membrane integrity. The data suggest that, for Ptr-dependent photoinitiated cell death, events related to mitochondrial failure precede those associated with the failure of the cell membrane.

引用

页码：862 / 866

页数：5

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