Regulation and functional expression of cinnamate 4-hydroxylase from parsley

被引：69

作者：

Koopmann, E ^{[1
]}

Logemann, E ^{[1
]}

Hahlbrock, K ^{[1
]}

机构：

[1] Max Planck Inst Zuchtungsforsch, Biochem Abt, D-50829 Cologne, Germany

来源：

PLANT PHYSIOLOGY | 1999年 / 119卷 / 01期

关键词：

D O I：

10.1104/pp.119.1.49

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A previously isolated parsley (Petroselinum crispum) cDNA with high sequence similarity to cinnamate 4-hydroxylase (C4H) cDNAs from several plant sources was expressed in yeast (Saccharomyces cerevisiae) containing a plant NADPH:cytochrome P450 oxidoreductase and verified as encoding a functional C4H (CYP73A10). Low genomic complexity and the occurrence of a single type of cDNA suggest the existence of only one C4H gene in parsley. The encoded mRNA and protein, in contrast to those of a functionally related NADPH:cytochrome P450 oxidoreductase, were strictly coregulated with phenylalanine ammonia-lyase mRNA and protein, respectively, as demonstrated by coinduction under various conditions and colocalization in situ in cross-sections from several different parsley tissues. These results support the hypothesis that the genes encoding the core reactions of phenylpropanoid metabolism form a tight regulatory unit.

引用

页码：49 / 55

页数：7

共 40 条

[1] STRUCTURAL AND CATALYTIC PROPERTIES OF THE 4 PHENYLALANINE AMMONIA-LYASE ISOENZYMES FROM PARSLEY (PETROSELINUM-CRISPUM NYM) [J].

APPERT, C ;

LOGEMANN, E ;

HAHLBROCK, K ;

SCHMID, J ;

AMRHEIN, N .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1994, 225 (01) :491-499

[2] HOST-PATHOGEN INTERACTIONS .10. FRACTIONATION AND BIOLOGICAL-ACTIVITY OF AN ELICITOR ISOLATED FROM MYCELIAL WALLS OF PHYTOPHTHORA-MEGASPERMA VAR SOJAE [J].

AYERS, AR ;

EBEL, J ;

VALENT, B ;

ALBERSHEIM, P .

PLANT PHYSIOLOGY, 1976, 57 (05) :760-765

[3] Extensive reprogramming of primary and secondary metabolism by fungal elicitor or infection in parsley cells [J].

Batz, O ;

Logemann, E ;

Reinold, S ;

Hahlbrock, K .

BIOLOGICAL CHEMISTRY, 1998, 379 (8-9) :1127-1135

[4] Cinnamate-4-hydroxylase expression in Arabidopsis - Regulation in response to development and the environment [J].

BellLelong, DA ;

Cusumano, JC ;

Meyer, K ;

Chapple, C .

PLANT PHYSIOLOGY, 1997, 113 (03) :729-738

[5] PHENYLALANINE AMMONIA-LYASE AND CINNAMIC ACID HYDROXYLASES AS ASSEMBLED CONSECUTIVE ENZYMES ON MICROSOMAL-MEMBRANES OF CUCUMBER COTYLEDONS - COOPERATION AND SUBCELLULAR-DISTRIBUTION [J].

CZICHI, U ;

KINDL, H .

PLANTA, 1977, 134 (02) :133-143

[6] STRUCTURE AND ELICITOR OR UV-LIGHT-STIMULATED EXPRESSION OF 2 4-COUMARATE-COA LIGASE GENES IN PARSLEY [J].

DOUGLAS, C ;

HOFFMANN, H ;

SCHULZ, W ;

HAHLBROCK, K .

EMBO JOURNAL, 1987, 6 (05) :1189-1195

[7]

Durst Francis, 1995, Drug Metabolism and Drug Interactions, V12, P171

[8]

Durst Francis, 1995, Drug Metabolism and Drug Interactions, V12, P189

[9] Cloning of wound-induced cytochrome P450 monooxygenases expressed in pea [J].

Frank, MR ;

Deyneka, JM ;

Schuler, MA .

PLANT PHYSIOLOGY, 1996, 110 (03) :1035-1046

[10] PURIFICATION AND IMMUNOCHARACTERIZATION OF A PLANT CYTOCHROME-P450 - THE CINNAMIC ACID 4-HYDROXYLASE [J].

GABRIAC, B ;

WERCKREICHHART, D ;

TEUTSCH, H ;

DURST, F .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1991, 288 (01) :302-309

← 1 2 3 4 →