Organ-specific distribution of ACE2 mRNA and correlating peptidase activity in rodents

被引：183

作者：

Gembardt, F

Sterner-Kock, A

Imboden, H

Spalteholz, M

Reibitz, F

Schultheiss, HP

Siems, WE

Walther, T ^{[1
]}

机构：

[1] Erasmus MC, Dept Pharmacol, NL-3015 GE Rotterdam, Netherlands

[2] Free Univ Berlin, Inst Vet Pathol, D-1000 Berlin, Germany

[3] Univ Bern, Inst Cell Biol, CH-3012 Bern, Switzerland

[4] Res Inst Mol Pharmacol, Berlin, Germany

来源：

PEPTIDES | 2005年 / 26卷 / 07期

关键词：

angiotensin-converting enzyme 2; enzyme activity; peptidase; tissue distribution; renin-angiotensin system;

D O I：

10.1016/j.peptides.2005.01.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Biochemical analysis revealed that angiotensin-converting enzyme related carboxy-peptidase (ACE2) cleaves angiotensin (Ana) 11 to Ang-(1-7), a heptapeptide identified as an endogenous ligand for the G protein-coupled receptor Mas. No data are currently available that systematically describe ACE2 distribution and activity in rodents. Therefore, we analyzed the ACE2 expression in different tissues of mice and rats on mRNA (RNase protection assay) and protein levels (immunohistochemistry, ACE2 activity, western blot). Although ACE2 mRNA in both investigated species showed the highest expression in the ileum, the mouse organ exceeded rat ACE2, as also demonstrated in the kidney and colon. Corresponding to mRNA, ACE2 activity was highest in the ileum and mouse kidney but weak in the rat kidney, which was also confirmed by immunohistochemistry. Contrary to mRNA, we found weak activity in the lung of both species. Our data demonstrate a tissue- and species-specific pattern for ACE2 under physiological conditions. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：1270 / 1277

页数：8

共 43 条

[21] Insights into angiotensin II receptor function through AT2 receptor knockout mice [J].