Monitoring poly(3-hydroxybutyrate) production in Cupriavidus necator DSM 428 (H 16) with Raman spectroscopy

被引：51

作者：

De Gelder, Joke ^{[1
]}

Willemse-Erix, Diana ^{[2
]}

Scholtes, Maarten J. ^{[2
]}

Sanchez, Jorge I. ^{[3
]}

Maquelin, Kees ^{[2
]}

Vandenabeele, Peter ^{[4
]}

De Boever, Patrick ^{[5
]}

Puppels, Gerwin J. ^{[2
,6
]}

Moens, Luc ^{[1
]}

De Vos, Paul ^{[7
]}

机构：

[1] Univ Ghent, Dept Analyt Chem, B-9000 Ghent, Belgium

[2] Univ Rotterdam, Med Ctr, Ctr Opt Diagnost & Therapy, Dept Dermatol Erasmus MC, NL-3015 GD Rotterdam, Netherlands

[3] Univ Ghent, Lab Microbial Ecol & Technol, B-9000 Ghent, Belgium

[4] Univ Ghent, Dept Archeol & Ancient Hist Europe, B-9000 Ghent, Belgium

[5] Belgian Nucl Res Ctr SCK CEN, Mol & Cellular Biol Lab, Inst Environm Hlth & Safety, B-2400 Mol, Belgium

[6] River Diagnost BV, NL-3015 GE Rotterdam, Netherlands

[7] Univ Ghent, Microbiol Lab, B-9000 Ghent, Belgium

来源：

ANALYTICAL CHEMISTRY | 2008年 / 80卷 / 06期

关键词：

D O I：

10.1021/ac702185d

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This study explored the potential of Raman spectroscopy for the analysis of poly(3-hydroxybutyrate) (PHB) in bacteria. PHB can be formed in large amounts by certain bacteria as a storage material and is of high importance for industrial biodegradable plastic production. Raman spectra were collected from Cupriavidus necator DSM 428 (H16), from its non-PHB-producing mutant strain C. necator DSM 541, and from pure PHB, in order to determine at which Raman shifts a contribution of PHB in bacterial spectra can be expected. The Raman band intensity at ca. 1734 cm(-1) appeared to be suitable for the monitoring of PHB production and consumption. These intensities were linearly related to the PHB concentration (mg L-1 culture) determined by parallel HPLC analysis. Therefore, Raman spectroscopy is considered as a fast and noninvasive technique for the determination and monitoring of the PHB content in bacteria.

引用

页码：2155 / 2160

页数：6

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