Functional analysis of pathogenicity proteins of the potato cyst nematode Globodera rostochiensis using RNAi

被引：109
作者：
Chen, Q
Rehman, S
Smant, G
Jones, JT [1 ]
机构：
[1] Scottish Crop Res Inst, Plant Pathogen Interact Programme, Dundee DD2 5DA, Scotland
[2] Univ Wageningen & Res Ctr, Dept Plant Sci, Nematol Lab, NL-6709 PD Wageningen, Netherlands
来源：
MOLECULAR PLANT-MICROBE INTERACTIONS | 2005年 / 18卷 / 07期
关键词：
amphid-secreted protein; cellulase; gene silencing; invasion;
D O I：
10.1094/MPMI-18-0621
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
RNA interference (RNAi) has been used widely as a tool for examining gene function and a method that allows its use with plant-parasitic nematodes recently has been described. Here, we use a modified method to analyze the function of secreted beta-1,4, endoglucanases of the potato cyst nematode Globodera rostochiensis, the first in vivo functional analysis of a pathogenicity protein of a plant-parasitic nematode. Knockout of the beta-1,4, endoglucanases reduced the ability of the nematodes to invade roots. We also use RNAi to show that gr-ams-1, a secreted protein of the main sense organs (the amphids), is essential for host location.
引用
收藏
页码：621 / 625
页数：5
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