Effect of hyaluronic acid molecular weight on the morphology of quantum dot-hyaluronic acid conjugates

被引：27

作者：

Kim, Jiseok ^{[1
]}

Park, Kitae ^{[1
]}

Hahn, Sei Kwang ^{[1
]}

机构：

[1] Pohang Univ Sci & Technol, Dept Mat Sci & Engn, Pohang 790784, South Korea

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2008年 / 42卷 / 01期

关键词：

quantum dots; hyaluronic acid; conjugates; TEM images; bio-imaging;

D O I：

10.1016/j.ijbiomac.2007.09.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The morphological analysis of novel quantum dot-hyaluronic acid (QDot-HA) conjugates was carried outwith a transmission electron microscope (TEM). Adipic acid dihydrazide-modified HA (HA-ADH) was synthesized and conjugated to quantum dots (QDots) having carboxyl terminal ligands which were activated with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysulfosuccinimide (sulfo-NHS). HA molecules with a molecular weight (MW) of 20 K, 234 K and 3000 K were used to investigate the effect of MW on the morphology of QDot-HA conjugates. The TEM micrographs of QDot-HA conjugates showed branched and multi-layered chain type morphology formed by inter- and intra-molecular conjugation of QDots to HA molecules. The size of QDot-HA conjugate increased with the MW of HA. QDot-HA conjugate could be successfully used for real-time bio-imaging of HA derivatives in nude mice. The novel QDot-HA conjugate will be further used to investigate the biological roles of HA with a different MW in the body. (c) 2007 Published by Elsevier B.V.

引用

页码：41 / 45

页数：5

共 31 条

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