Cell-laden microengineered pullulan methacrylate hydrogels promote cell proliferation and 3D cluster formation

被引：103

作者：

Bae, Hojae ^{[1
,2
]}

Ahari, Amir F. ^{[1
,2
]}

Shin, Hyeongho ^{[1
,3
]}

Nichol, Jason W. ^{[1
,2
]}

Hutson, Che B. ^{[1
,2
]}

Masaeli, Mahdokht ^{[1
,2
]}

Kim, Su-Hwan ^{[1
,2
,4
]}

Aubin, Hug ^{[1
,2
]}

Yamanlar, Seda ^{[1
,2
]}

Khademhosseini, Ali ^{[1
,2
]}

机构：

[1] Harvard Univ, Sch Med, Brigham & Womens Hosp, Ctr Biomed Engn,Dept Med, Cambridge, MA 02139 USA

[2] MIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USA

[3] MIT, Dept Mat Sci & Engn, Cambridge, MA 02139 USA

[4] Korea Univ, Coll Hlth Sci, Dept Biomed Engn, Seoul 136703, South Korea

来源：

SOFT MATTER | 2011年 / 7卷 / 05期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

PHOTOCROSSLINKABLE HYALURONIC-ACID; EXTRACELLULAR-MATRIX; ENZYMATIC DEGRADATION; DERIVATIVES; ENCAPSULATION; FABRICATION; MICROGELS; CULTURE;

D O I：

10.1039/c0sm00697a

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The ability to encapsulate cells in three-dimensional (3D) environments is potentially of benefit for tissue engineering and regenerative medicine. In this paper, we introduce pullulan methacrylate (PulMA) as a promising hydrogel platform for creating cell-laden microscale tissues. The hydration and mechanical properties of PulMA were demonstrated to be tunable through modulation of the degree of methacrylation and gel concentration. Cells encapsulated in PulMA exhibited excellent viability. Interestingly, while cells did not elongate in PulMA hydrogels, cells proliferated and organized into clusters, the size of which could be controlled by the hydrogel composition. By mixing with gelatin methacrylate (GelMA), the biological properties of PulMA could be enhanced as demonstrated by cells readily attaching to, proliferating, and elongating within the PulMA/GelMA composite hydrogels. These data suggest that PulMA hydrogels could be useful for creating complex, cell-responsive microtissues, especially for applications that require controlled cell clustering and proliferation.

引用

页码：1903 / 1911

页数：9

共 47 条

[1] Directed 3D cell alignment and elongation in microengineered hydrogels [J].