Effects of Tert-Butylhydroquinone on Intestinal Inflammatory Response and Apoptosis following Traumatic Brain Injury in Mice

被引:45
作者
Jin, Wei [1 ]
Ni, Hongbin [1 ]
Dai, Yuxiang [1 ]
Wang, Handong [2 ]
Lu, Tianyu [1 ]
Wu, Jun [1 ]
Jiang, Jian [1 ]
Liang, Weibang [1 ]
机构
[1] Nanjing Univ, Dept Neurosurg, Drum Tower Hosp, Sch Med, Nanjing 210008, Jiangsu, Peoples R China
[2] Nanjing Univ, Dept Neurosurg, Jinling Hosp, Sch Med, Nanjing 210002, Jiangsu, Peoples R China
关键词
NF-KAPPA-B; NRF2-DRIVEN ANTIOXIDANT RESPONSE; NRF2 ENHANCES SUSCEPTIBILITY; GLUTATHIONE-S-TRANSFERASE; OXIDATIVE STRESS; TRANSCRIPTION FACTOR; NRF2-DEFICIENT MICE; PIVOTAL ROLE; INDUCTION; PROTECTION;
D O I
10.1155/2010/502564
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Traumatic brain injury (TBI) can induce intestinal inflammatory response and mucosal injury. Antioxidant transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2) has been shown in our previous studies to prevent oxidative stress and inflammatory response in gut after TBI. The objective of this study was to test whether tert-butylhydroquinone (tBHQ), an Nrf2 inducer, can protect against TBI-induced intestinal inflammatory response and mucosal injury in mice. Adult male ICR mice were randomly divided into three groups: (1) sham + vehicle group, (2) TBI + vehicle group, and (3) TBI + tBHQ group (n = 12 per group). Closed head injury was adopted using Hall's weight-dropping method. Intestinal mucosa apoptosis and inflammatory-related factors, such as nuclear factor kappa B (NF-kappa B), tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6) and intercellular adhesion molecule-1 (ICAM-1), were investigated at 24 h after TBI. As a result, we found that oral treatment with 1% tBHQ prior to TBI for one week markedly decreased NF-kappa B activation, inflammatory cytokines production, and ICAM-1 expression in the gut. Administration of tBHQ also significantly attenuated TBI-induced intestinal mucosal apoptosis. The results of the present study suggest that tBHQ administration could suppress the intestinal inflammation and reduce the mucosal damage following TBI.
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页数:7
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