VDAC2 (porin-2) expression pattern and localization in the bovine testis

被引:49
作者
Hinsch, KD [1 ]
Asmarinah [1 ]
Hinsch, E [1 ]
Konrad, L [1 ]
机构
[1] Univ Giessen, Dept Dermatol & Androl, D-35392 Giessen, Germany
来源
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 2001年 / 1518卷 / 03期
关键词
voltage anion dependent channel 1; voltage anion dependent channel 2; gene expression; spermatocyte; spermatozoon;
D O I
10.1016/S0167-4781(01)00199-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this study, sequencing of voltage-dependent anion channel 2 (VDAC2, porin-2) cDNA from bovine testis is reported. High identity to the murine, rabbit, and human subtypes at both the nucleotide and amino acid levels is demonstrated. mRNA analysis revealed expression of VDAC2 in bovine testis, whereas high levels of VDAC2 proteins were found in late spermatocytes, spermatids, and spermatozoa. In contrast, VDAC1 (porin-1) is exclusively localized in Sertoli cells. The possible role of testicular VDAC2 in providing energy metabolites and in germ cell apoptosis is discussed. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:329 / 333
页数:5
相关论文
共 19 条
  • [1] Porin is present in the plasma membrane where it is concentrated in caveolae and caveolae-related domains
    Bàthori, G
    Parolini, I
    Tombola, F
    Szabò, I
    Messina, A
    Oliva, M
    De Pinto, V
    Lisanti, M
    Sargiacomo, M
    Zoratti, M
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (42) : 29607 - 29612
  • [2] PERMEATION OF HYDROPHILIC SOLUTES THROUGH MITOCHONDRIAL OUTER MEMBRANES - REVIEW ON MITOCHONDRIAL PORINS
    BENZ, R
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON BIOMEMBRANES, 1994, 1197 (02): : 167 - 196
  • [3] BLACHLYDYSON E, 1993, J BIOL CHEM, V268, P1835
  • [4] Evidence for secretory pathway localization of a voltage-dependent anion channel isoform
    Buettner, R
    Papoutsoglou, G
    Scemes, E
    Spray, DC
    Dermietzel, R
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (07) : 3201 - 3206
  • [5] The mitochondrial permeability transition pore and its role in cell death
    Crompton, M
    [J]. BIOCHEMICAL JOURNAL, 1999, 341 : 233 - 249
  • [6] Bax, Bid and the permeabilization of the mitochondrial outer membrane in apoptosis
    Crompton, M
    [J]. CURRENT OPINION IN CELL BIOLOGY, 2000, 12 (04) : 414 - 419
  • [7] The tissue-specific, alternatively spliced single ATG exon of the type 3 voltage-dependent anion channel gene does not create a truncated protein isoform in vivo
    Decker, WK
    Craigen, WJ
    [J]. MOLECULAR GENETICS AND METABOLISM, 2000, 70 (01) : 69 - 74
  • [8] Furuchi T, 1996, DEVELOPMENT, V122, P1703
  • [9] IDENTIFICATION OF MOUSE ZP3 PROTEIN IN MAMMALIAN OOCYTES WITH ANTISERA AGAINST SYNTHETIC ZP3 PEPTIDES
    HINSCH, KD
    HINSCH, E
    MEINECKE, B
    TOPFERPETERSEN, E
    PFISTERER, S
    SCHILL, WB
    [J]. BIOLOGY OF REPRODUCTION, 1994, 51 (02) : 193 - 204
  • [10] The release of cytochrome c from mitochondria: A primary site for Bcl-2 regulation of apoptosis
    Kluck, RM
    BossyWetzel, E
    Green, DR
    Newmeyer, DD
    [J]. SCIENCE, 1997, 275 (5303) : 1132 - 1136