Engineering a 2D protein-DNA crystal

被引：148
作者：
Malo, J
Mitchell, JC
Vénien-Bryan, C
Harris, JR
Wille, H
Sherratt, DJ
Turberfield, AJ
机构：
[1] Univ Oxford, Dept Phys, Clarendon Lab, Oxford OX1 3PU, England
[2] Univ Oxford, Dept Biochem, Lab Mol Biophys, Oxford OX1 3QU, England
[3] Johannes Gutenberg Univ Mainz, Inst Zool, D-55099 Mainz, Germany
[4] Univ Calif San Francisco, Dept Neurol, San Francisco, CA 94143 USA
[5] Univ Calif San Francisco, Inst Neurodegenerat Dis, San Francisco, CA 94143 USA
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 20期
基金：
英国工程与自然科学研究理事会; 英国惠康基金;
关键词：
DNA; electron microscopy; nanostructures; proteins; self-assembly;
D O I：
10.1002/anie.200463027
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
(Figure Presented) Weaving with DNA: A DNA-binding protein was used to control the structure of a self-assembled 2D crystal. In the absence of protein, four oligonucleotides hybridize to form a Kagome lattice of interwoven double helices with p3 symmetry (see image). Addition of protein RuvA during assembly changes the symmetry and connectivity to give a DNA-protein crystal with an approximately square unit cell. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码：3057 / 3061
页数：5
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