The Art of MicroRNA Research

被引：478
|
作者：
van Rooij, Eva [1 ]
机构：
[1] miRagen Therapeut Inc, Boulder, CO 80301 USA
来源：
CIRCULATION RESEARCH | 2011年 / 108卷 / 02期
关键词：
miRNA; cardiovascular; research methods; IN-SITU DETECTION; HEPATITIS-C VIRUS; SMALL RNAS; POSTTRANSCRIPTIONAL REGULATION; CARDIAC-HYPERTROPHY; PROTEIN-SYNTHESIS; EXPRESSION; GENES; DICER; TARGETS;
D O I：
10.1161/CIRCRESAHA.110.227496
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Originally identified as moderate biological modifiers, microRNAs have recently emerged as powerful regulators of diverse cellular processes with especially important roles in disease and tissue remodeling. The rapid pace of studies on microRNA regulation and function necessitates the development of suitable techniques for measuring and modulating microRNAs in different model systems. This review summarizes experimental strategies for microRNA research and highlights the strengths and weaknesses of different approaches. The development of more specific and sensitive assays will further illuminate the biology behind microRNAs and will advance opportunities to safely pursue them as therapeutic modalities. (Circ Res. 2011;108:219-234.)
引用
收藏
页码：219 / 234
页数：16
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