The differential activation of intracellular signaling pathways confers the permissiveness of embryonic stem cell derivation from different mouse strains

被引：19
|
作者：
Ohtsuka, Satoshi [1 ]
Niwa, Hitoshi [1 ,2 ,3 ]
机构：
[1] RIKEN, CDB, Lab Pluripotent Stem Cell Studies, Chuo Ku, Kobe, Hyogo 6500047, Japan
[2] Japan Sci & Technol Agcy, CREST, Saitama 3320012, Japan
[3] Kobe Univ, Grad Sch Med, Lab Dev & Regenerat Med, Chuo Ku, Kobe, Hyogo 6500017, Japan
来源：
DEVELOPMENT | 2015年 / 142卷 / 03期
基金：
日本科学技术振兴机构;
关键词：
LIF signaling; MAP kinase; Stat3; Embryonic stem cell; Signal responsiveness; SELF-RENEWAL; ES CELLS; RAT BLASTOCYSTS; ESTABLISHMENT; STAT3; LINES; PLURIPOTENCY; FGF; CULTURE; PROTEIN;
D O I：
10.1242/dev.112375
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The requirement of leukemia inhibitory factor (LIF) for the establishment and maintenance of mouse embryonic stem cells (ESCs) depends on the genetic background of the ESC origin. To reveal the molecular basis of the strain-dependent function of LIF, we compared the activation of the intracellular signaling pathways downstream of LIF in ESCs with different genetic backgrounds. We found that the JAK-Stat3 pathway was dominantly activated in ESCs derived from 'permissive' mouse strains (129Sv and C57BL6), whereas the MAP kinase pathway was hyperactivated in ESCs from 'non-permissive' strains (NOD, CBA and FVB). Artificial activation of Stat3 supported stable self-renewal of ESCs from non-permissive strains. These data suggest that the difference in the balance between the two intracellular signaling pathways underlies the differential response to LIF.
引用
收藏
页码：431 / 437
页数：7
相关论文
共 18 条
  • [1] Derivation and characterization of mouse embryonic stem cells from permissive and nonpermissive strains
    Czechanski, Anne
    Byers, Candice
    Greenstein, Ian
    Schrode, Nadine
    Donahue, Leah Rae
    Hadjantonakis, Anna-Katerina
    Reinholdt, Laura G.
    NATURE PROTOCOLS, 2014, 9 (03) : 559 - 574
  • [2] Optimization of Protocols for Derivation of Mouse Embryonic Stem Cell Lines from Refractory Strains, Including the Non Obese Diabetic Mouse
    Davies, Timothy J.
    Fairchild, Paul J.
    STEM CELLS AND DEVELOPMENT, 2012, 21 (10) : 1688 - 1700
  • [3] Activation of Wnt/β-Catenin Signaling Pathway Enhances the Derivation of Buffalo (Bubalus bubalis) Embryonic Stem Cell-Like Cells
    Deng, Yanfei
    Lao, Yanping
    Ruan, Qiuyan
    Zhang, Jun
    Luo, Chan
    Shi, Deshun
    Lu, Fenghua
    CELLULAR REPROGRAMMING, 2020, 22 (04) : 217 - 225
  • [4] Derivation of embryonic stem cells from wild-derived mouse strains by nuclear transfer using peripheral blood cells
    Watanabe, Naomi
    Hirose, Michiko
    Hasegawa, Ayumi
    Mochida, Keiji
    Ogura, Atsuo
    Inoue, Kimiko
    SCIENTIFIC REPORTS, 2023, 13 (01)
  • [5] Derivation of stable embryonic stem cell-like, but transcriptionally heterogenous, induced pluripotent stem cells from non-permissive mouse strains
    Garbutt, Tiffany A.
    Konganti, Kranti
    Konneker, Thomas
    Hillhouse, Andrew
    Phelps, Drake
    Jones, Alexis
    Aylor, David
    Threadgill, David W.
    MAMMALIAN GENOME, 2020, 31 (9-12) : 263 - 286
  • [6] Pluripotin Combined with Leukemia Inhibitory Factor Greatly Promotes the Derivation of Embryonic Stem Cell Lines from Refractory Strains
    Yang, Weifeng
    Wei, Wei
    Shi, Cheng
    Zhu, Jinliang
    Ying, Wenqin
    Shen, Yan
    Ye, Xin
    Fang, Lingling
    Duo, Shuguang
    Che, Jie
    Shen, Huan
    Ding, Sheng
    Deng, Hongkui
    STEM CELLS, 2009, 27 (02) : 383 - 389
  • [7] Cross-activation of FGF, NODAL, and WNT pathways constrains BMP-signaling-mediated induction of the totipotent state in mouse embryonic stem cells
    Meharwade, Thulaj
    Joumier, Loick
    Parisotto, Maxime
    Huynh, Vivian
    da Rocha, Edroaldo Lummertz
    Malleshaiah, Mohan
    CELL REPORTS, 2023, 42 (05):
  • [8] Metastable primordial germ cell-like state induced from mouse embryonic stem cells by Akt activation
    Yamano, Noriko
    Kimura, Tohru
    Watanabe-Kushima, Shoko
    Shinohara, Takashi
    Nakano, Toru
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2010, 392 (03) : 311 - 316
  • [9] Influence of E-Cadherin-Mediated Cell Adhesion on Mouse Embryonic Stem Cells Derivation from Isolated Blastomeres
    Gonzalez, Sheyla
    Ibanez, Elena
    Santalo, Josep
    STEM CELL REVIEWS AND REPORTS, 2011, 7 (03) : 494 - 505
  • [10] Wnt/β-catenin and LIF-Stat3 signaling pathways converge on Sp5 to promote mouse embryonic stem cell self-renewal
    Ye, Shoudong
    Zhang, Dongming
    Cheng, Fei
    Wilson, Daniel
    Mackay, Jeffrey
    He, Kan
    Ban, Qian
    Lv, Feng
    Huang, Saifei
    Liu, Dahai
    Ying, Qi-Long
    JOURNAL OF CELL SCIENCE, 2016, 129 (02) : 269 - 276
12