Long-term in vitro cultivation of Borrelia miyamotoi

被引:29
作者
Margos, Gabriele [1 ,2 ]
Stockmeier, Sylvia [1 ,2 ]
Hizo-Teufel, Cecilia [1 ,2 ]
Hepner, Sabrina [2 ]
Fish, Durland [3 ]
Dautel, Hans [4 ]
Sing, Andreas [1 ]
Dzaferovic, Eldina [2 ]
Rieger, Melissa [2 ,5 ]
Jungnick, Sabrina [2 ,5 ]
Binder, Katrin [2 ]
Straubinger, Reinhard K. [5 ]
Fingerle, Volker [1 ,2 ]
机构
[1] Bavarian Hlth & Food Safety Author, D-85764 Oberschleissheim, Germany
[2] German Natl Reference Ctr Borrelia, Oberschleissheim, Germany
[3] Yale Univ, Sch Publ Hlth, New Haven, CT USA
[4] IS Insect Serv GmbH, Berlin, Germany
[5] Univ Munich, Munich, Germany
关键词
Borrelia miyamotoi; Relapsing fever spirochetes; In vitro cultivation; LYME-DISEASE; MENINGOENCEPHALITIS; HUMANS; VECTOR; TICK;
D O I
10.1016/j.ttbdis.2014.12.001
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Borrelia are fastidious bacteria some of which are difficult to grow in vitro. Here, we report a method for successful continuous in vitro cultivation of the emerging pathogen Borrelia miyamotoi. The type and quantity of serum as well as the atmosphere were critical for successful in vitro cultivation. Optimal growth was achieved using 50% pooled human serum and an atmosphere of 6% CO2. (C) 2014 Elsevier GmbH. All rights reserved.
引用
收藏
页码:181 / 184
页数:4
相关论文
共 50 条
  • [21] Identification of immunoreactive linear epitopes of Borrelia miyamotoi
    Tokarz, Rafal
    Tagliafierro, Teresa
    Caciula, Adrian
    Mishra, Nischay
    Thakkar, Riddhi
    Chauhan, Lokendra, V
    Sameroff, Stephen
    Delaney, Shannon
    Wormser, Gary P.
    Marques, Adriana
    Lipkin, W. Ian
    TICKS AND TICK-BORNE DISEASES, 2020, 11 (01)
  • [22] Borrelia miyamotoi in host-seeking Ixodes ricinus ticks in England
    Hansford, K. M.
    Fonville, M.
    Jahfari, S.
    Sprong, H.
    Medlock, J. M.
    EPIDEMIOLOGY AND INFECTION, 2015, 143 (05) : 1079 - 1087
  • [23] A comparison of horizontal and transovarial transmission efficiency of Borrelia miyamotoi by Ixodes scapularis
    Lynn, Geoffrey E.
    Breuner, Nicole E.
    Hojgaard, Andrias
    Oliver, Jonathan
    Eisen, Lars
    Eisen, Rebecca J.
    TICKS AND TICK-BORNE DISEASES, 2022, 13 (05)
  • [24] Isolation of Borrelia miyamotoi and other Borreliae using a modified BSK medium
    Replogle, Adam J.
    Sexton, Christopher
    Young, John
    Kingry, Luke C.
    Schriefer, Martin E.
    Dolan, Marc
    Johnson, Tammi L.
    Connally, Neeta P.
    Padgett, Kerry A.
    Petersen, Jeannine M.
    SCIENTIFIC REPORTS, 2021, 11 (01)
  • [25] Ticks, fleas and rodent-hosts analyzed for the presence of Borrelia miyamotoi in Slovakia: the first record of Borrelia miyamotoi in a Haemaphysalis inermis tick
    Heglasova, Ivana
    Rudenko, Natalie
    Golovchenko, Maryna
    Zubrikova, Dana
    Miklisova, Dana
    Stanko, Michal
    TICKS AND TICK-BORNE DISEASES, 2020, 11 (05)
  • [26] Detection of Borrelia burgdorferi Sensu Lato and Relapsing Fever Borrelia in Feeding Ixodes Ticks and Rodents in Sarawak, Malaysia: New Geographical Records of Borrelia yangtzensis and Borrelia miyamotoi
    Lau, Alice C. C.
    Qiu, Yongjin
    Moustafa, Mohamed Abdallah Mohamed
    Nakao, Ryo
    Shimozuru, Michito
    Onuma, Manabu
    Mohd-Azlan, Jayasilan
    Tsubota, Toshio
    PATHOGENS, 2020, 9 (10): : 1 - 18
  • [27] Associations of passerine birds, rabbits, and ticks with Borrelia miyamotoi and Borrelia andersonii in Michigan, USA
    Hamer, Sarah A.
    Hickling, Graham J.
    Keith, Rich
    Sidge, Jennifer L.
    Walker, Edward D.
    Tsao, Jean I.
    PARASITES & VECTORS, 2012, 5
  • [28] Distribution and survival of Borrelia miyamotoi in human blood components
    Thorp, Aaron M.
    Tonnetti, Laura
    TRANSFUSION, 2016, 56 (03) : 705 - 711
  • [29] A new Borrelia on the block: Borrelia miyamotoi - a human health risk?
    Cutler, Sally
    Vayssier-Taussat, Muriel
    Estrada-Pena, Agustin
    Potkonjak, Aleksandar
    Mihalca, Andrei Daniel
    Zeller, Herve
    EUROSURVEILLANCE, 2019, 24 (18): : 12 - 25
  • [30] Borrelia miyamotoi in vectors and hosts in The Netherlands
    Wagemakers, Alex
    Jahfari, Seta
    de Weyer, Bob
    Spanjaard, Lodewijk
    Starink, Markus V.
    de Vries, Henry J. C.
    Sprong, Hein
    Hoviusf, Joppe W.
    TICKS AND TICK-BORNE DISEASES, 2017, 8 (03) : 370 - 374