Long-term in vitro cultivation of Borrelia miyamotoi

被引:29
作者
Margos, Gabriele [1 ,2 ]
Stockmeier, Sylvia [1 ,2 ]
Hizo-Teufel, Cecilia [1 ,2 ]
Hepner, Sabrina [2 ]
Fish, Durland [3 ]
Dautel, Hans [4 ]
Sing, Andreas [1 ]
Dzaferovic, Eldina [2 ]
Rieger, Melissa [2 ,5 ]
Jungnick, Sabrina [2 ,5 ]
Binder, Katrin [2 ]
Straubinger, Reinhard K. [5 ]
Fingerle, Volker [1 ,2 ]
机构
[1] Bavarian Hlth & Food Safety Author, D-85764 Oberschleissheim, Germany
[2] German Natl Reference Ctr Borrelia, Oberschleissheim, Germany
[3] Yale Univ, Sch Publ Hlth, New Haven, CT USA
[4] IS Insect Serv GmbH, Berlin, Germany
[5] Univ Munich, Munich, Germany
关键词
Borrelia miyamotoi; Relapsing fever spirochetes; In vitro cultivation; LYME-DISEASE; MENINGOENCEPHALITIS; HUMANS; VECTOR; TICK;
D O I
10.1016/j.ttbdis.2014.12.001
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Borrelia are fastidious bacteria some of which are difficult to grow in vitro. Here, we report a method for successful continuous in vitro cultivation of the emerging pathogen Borrelia miyamotoi. The type and quantity of serum as well as the atmosphere were critical for successful in vitro cultivation. Optimal growth was achieved using 50% pooled human serum and an atmosphere of 6% CO2. (C) 2014 Elsevier GmbH. All rights reserved.
引用
收藏
页码:181 / 184
页数:4
相关论文
共 50 条
  • [1] In Vitro Susceptibility of the Relapsing-Fever Spirochete Borrelia miyamotoi to Antimicrobial Agents
    Koetsveld, Joris
    Draga, Ronald O. P.
    Wagemakers, Alex
    Manger, Annemijn
    Oei, Anneke
    Visser, Caroline E.
    Hovius, Joppe W.
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2017, 61 (09)
  • [2] Human Infections with Borrelia miyamotoi, Japan
    Sato, Kozue
    Takano, Ai
    Konnai, Satoru
    Nakao, Minoru
    Ito, Takuya
    Koyama, Kojiro
    Kaneko, Minoru
    Ohnishi, Makoto
    Kawabata, Hiroki
    EMERGING INFECTIOUS DISEASES, 2014, 20 (08) : 1391 - 1393
  • [3] Borrelia miyamotoi is widespread in Ixodes ricinus ticks in southern Norway
    Kjelland, Vivian
    Rollum, Rikke
    Korslund, Lars
    Slettan, Audun
    Tveitnes, Dag
    TICKS AND TICK-BORNE DISEASES, 2015, 6 (04) : 516 - 521
  • [4] Borrelia miyamotoi Disease Neither Lyme Disease Nor Relapsing Fever
    Telford, Sam R., III
    Goethert, Heidi K.
    Molloy, Philip J.
    Berardi, Victor P.
    Chowdri, Hanumara Ram
    Gugliotta, Joseph L.
    Lepore, Timothy J.
    CLINICS IN LABORATORY MEDICINE, 2015, 35 (04) : 867 - +
  • [5] In Vitro Antimicrobial Susceptibility of Clinical Isolates of Borrelia miyamotoi
    Koetsveld, Joris
    Manger, Annemijn
    Hoornstra, Dieuwertje
    Draga, Ronald O.
    Oei, Anneke
    Kolyasnikova, Nadezhda M.
    Toporkova, Marina G.
    Sarksyan, Denis S.
    Wagemakers, Alex
    Platonov, Alexander E.
    Hovius, Joppe W.
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2018, 62 (07)
  • [6] Borrelia miyamotoi: A Comprehensive Review
    Cleveland, Dawn W. W.
    Anderson, Cassidy C. C.
    Brissette, Catherine A. A.
    PATHOGENS, 2023, 12 (02):
  • [7] First report of Borrelia miyamotoi in an Ixodes ricinus tick in Augsburg, Germany
    Page, Sharon
    Daschkin, Christina
    Anniko, Sirli
    Krey, Viktoria
    Nicolaus, Carsten
    Maxeiner, Horst-Guenter
    EXPERIMENTAL AND APPLIED ACAROLOGY, 2018, 74 (02) : 191 - 199
  • [8] Development and optimization of an in vitro cultivation protocol allows for isolation of Borrelia miyamotoi from patients with hard tick-borne relapsing fever
    Koetsveld, J.
    Kolyasnikova, N. M.
    Wagemakers, A.
    Toporkova, M. G.
    Sarksyan, D. S.
    Oei, A.
    Platonov, A. E.
    Hovius, J. W.
    CLINICAL MICROBIOLOGY AND INFECTION, 2017, 23 (07) : 480 - 484
  • [9] Borrelia miyamotoi infection in nature and in humans
    Krause, P. J.
    Fish, D.
    Narasimhan, S.
    Barbour, A. G.
    CLINICAL MICROBIOLOGY AND INFECTION, 2015, 21 (07) : 631 - 639
  • [10] Borrelia miyamotoi: A Lesson in Disease Discovery
    Branda, John A.
    Rosenberg, Eric S.
    ANNALS OF INTERNAL MEDICINE, 2013, 159 (01) : 61 - +