Development of 105 SNP markers in endangered turtle species Pelodiscus sinensis using RAD-seq

被引:1
作者
Xie, Qiming [1 ]
Liu, Fan [1 ]
Zhang, Jilong [1 ]
Li, Xilei [1 ]
Chen, Tiantian [1 ]
Fang, Guoxia [2 ]
Ma, Rensheng [2 ]
Su, Shiping [1 ]
机构
[1] Anhui Agr Univ, Coll Anim Sci & Technol, 130 Changjiang West Rd, Hefei 230036, Anhui, Peoples R China
[2] Feixi Cty Anim Dis Prevent & Control Ctr, Hefei 230036, Anhui, Peoples R China
关键词
Pelodiscus sinensis; RAD-seq; SNP; Conservation genetics; GENETIC DIVERSITY;
D O I
10.1007/s12686-021-01238-z
中图分类号
X176 [生物多样性保护];
学科分类号
090705 ;
摘要
Restriction site-associated DNA sequencing (RAD-seq) was used to develop single nucleotide polymorphisms (SNP) marker for the Pelodiscus sinensis, which is a commercially important species in China. A total of 105 high-quality SNP markers were identified. The genetic statistics showed that the minor allele frequency varied from 0.0571 to 0.3382 and the observed heterozygosity and the expected heterozygosity ranged from 0.1143 to 0.6765 and 0.1078 to 0.4477, respectively. These novel SNP markers will serve as a valuable genetic tool for future conservation and population genetics studies for P. sinensis.
引用
收藏
页码:27 / 30
页数:4
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