Curcumin Alleviates Cerebral Ischemia-reperfusion Injury by Inhibiting NLRP1-dependent Neuronal Pyroptosis

被引：48

作者：

Huang, Lifa ^{[1
]}

Li, Xu ^{[1
]}

Liu, Yajun ^{[1
]}

Liang, Xiaolong ^{[1
]}

Ye, Hui ^{[1
]}

Yang, Chao ^{[1
]}

Hua, Lin ^{[2
]}

Zhang, Xin ^{[1
]}

机构：

[1] Zhejiang Chinese Med Univ, Affiliated Hosp 1, Dept Neurosurg, Hangzhou 310006, Zhejiang, Peoples R China

[2] Zhejiang Chinese Med Univ, Clin Med Coll 1, Hangzhou 310053, Zhejiang, Peoples R China

来源：

CURRENT NEUROVASCULAR RESEARCH | 2021年 / 18卷 / 02期

关键词：

Cerebral ischemia-reperfusion injury; curcumin; pyroptosis; NLRP1; blood reperfusion; brain; OXIDATIVE STRESS; LUNG INJURY; PROTECTS; APOPTOSIS; AUTOPHAGY; DISTINCT; TISSUES; STROKE; BRAIN;

D O I：

10.2174/1567202618666210607150140

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

10.2174/1567202618666210607150140 Abstract: Background: Cerebral ischemia-reperfusion injury is caused by a blood reperfusion injury in the ischemic brain and usually occurs in the treatment stage of ischemic disease, which can aggravate brain tissue injury. Objective: Curcumin was reported to exert a good therapeutic effect on neural cells against ischemia-reperfusion injury, However, the mechanism is not clear. Methods: In this study, Oxygen-Glucose Deprivation (OGD) model of fetal rat cerebral cortical neurons and the Middle Cerebral Artery Occlusion (MCAO) model of rats were employed to mimic cerebral ischemia-reperfusion injury in vitro and in vivo, respectively. Results: We confirmed that curcumin has a promotive effect on neuronal proliferation and an inhibitory effect on neuronal pyroptosis. Furthermore, we found that curcumin could improve cerebral infarction. The results of western blotting showed that curcumin down-regulated the expression of nucleotide-binding oligomerization domain-containing protein-, leucine-rich repeats-, and pyrin domain-containing protein 1 (NLRP1), cysteinyl aspartate-specific protease 1 (caspase-1), gasdermin D (GSDMD), IL-1 beta, IL-6, TNF-alpha, and iNOS proteins in OGD and MCAO models. NLRP1-dependent neuronal pyroptosis played an important role in cerebral ischemia-reperfusion injury. Conclusion: Curcumin could effectively inhibit NLRP1-dependent neuronal pyroptosis by suppressing the p38 MAPK pathway and therefore exerted neuroprotective effects against cerebral ischemia-reperfusion injury.

引用

页码：189 / 196

页数：8

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