Dynamic Chemistry of Disulfide Terminated Oligonucleotides in Duplexes and Double-Crossover Tiles

被引:22
作者
De Stefano, Mattia [1 ,2 ]
Gothelf, Kurt Vesterager [1 ,2 ]
机构
[1] Danish Natl Res Fdn, Ctr DNA Nanotechnol, Dept Chem, Gustav Wieds Vej 14, DK-8000 Aarhus C, Denmark
[2] INANO, Gustav Wieds Vej 14, DK-8000 Aarhus C, Denmark
基金
新加坡国家研究基金会;
关键词
disulfides; DNA structures; dynamic combinatorial chemistry; nucleic acids; reduction; DNA NANOSTRUCTURES; NUCLEIC-ACIDS; ORIGAMI; REACTIVITY; MOLECULES; LIGATION; PROTEINS; LINKING; SHAPES; STATE;
D O I
10.1002/cbic.201600076
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Designed nanostructures formed by self-assembly of multiple DNA strands suffer from low stability at elevated temperature and under other denaturing conditions. Here, we propose a method for covalent coupling of DNA strands in such structures by the formation of disulfide bonds; this allows disassembly of the structure under reducing conditions. The dynamic chemistry of disulfides and thiols was applied to crosslink DNA strands with terminal disulfide modifications. The formation of disulfide-linked DNA duplexes consisting of three strands is demonstrated, as well as a more-complex DNA double-crossover tile. All the strands in the fully disulfide-linked structures are covalently and geometrically interlocked, and it is demonstrated that the structures are stable under heating and in the presence of denaturants. Such a reversible system can be exploited in applications where higher DNA stability is needed only temporarily, such as delivery of cargoes to cells by DNA nanostructures.
引用
收藏
页码:1122 / 1126
页数:5
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