Arterial hypertension exacerbates oxidative stress in early diabetic retinopathy

被引：32

作者：

Pinto, Camila C. ^{[1
]}

Silva, Kamila C. ^{[1
]}

Biswas, Subrata K. ^{[1
]}

Martins, Natassia ^{[1
]}

De Faria, Jose B. Lopes ^{[1
]}

De Faria, Jacqueline M. Lopes ^{[1
]}

机构：

[1] Univ Estadual Campinas, Fac Med Sci, Renal Pathophysiol Lab, Dept Internal Med, BR-13084971 Campinas, SP, Brazil

来源：

FREE RADICAL RESEARCH | 2007年 / 41卷 / 10期

基金：

巴西圣保罗研究基金会;

关键词：

diabetic retinopathy; hypertension; oxidative stress; superoxide; nitrotyrosine; 8-hydroxy; 2'-deoxyguanosine; glutathione;

D O I：

10.1080/10715760701632816

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The present study was undertaken to investigate the redox status in the retina of an experimental model that combines hypertension and diabetes. Spontaneously hypertensive rats (SHR) and their control Wystar Kyoto (WKY) rats were rendered diabetic and, after 20 days, the rats were sacrificed and the retinas collected. The superoxide production was higher in diabetic than in control WKY (p < 0.03) and SHR rats showed elevated superoxide production compared with WKY groups (p < 0.009). The glutathione antioxidant system was diminished only in diabetic SHR (p < 0.04). Tirosyne nitration was higher in diabetic WKYand control SHR compared with control WKY (p < 0.03), and further increment was observed in diabetic SHR (p < 0.02). The DNA damage estimated by immunohystochemistry for 8-OHdG was higher in control SHR than in WKY, mainly in diabetic SHR (p < 0.0001). Hypertension aggravates oxidative-induced cytotoxicity in diabetic retina due to increasing of superoxide production and impairment of antioxidative system.

引用

页码：1151 / 1158

页数：8

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