Determination of ceramides and diglycerides by the diglyceride kinase assay

被引：81

作者：

Bielawska, A ^{[1
]}

Perry, DK ^{[1
]}

Hannun, YA ^{[1
]}

机构：

[1] Med Univ S Carolina, Dept Biochem & Mol Biol, Charleston, SC 29425 USA

来源：

ANALYTICAL BIOCHEMISTRY | 2001年 / 298卷 / 02期

关键词：

ceramides; diglycerides; DG kinase;

D O I：

10.1006/abio.2001.5342

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This review will discuss the utilization of the diglyceride (DG) kinase assay as an analytical method that allows the simultaneous quantitation of DG and ceramide from cell and tissue samples. This enzymatic approach is sensitive, quantitative, and linear over a broad range (20 pmol to 20-25 nmol) for both classes of lipids. It is also practical in that it can be applied to crude lipid extracts and used to process many samples (up to 100) concurrently. However, it has become apparent that this assay has not been conducted optimally, primarily as a result of lack of adherence to its basic principles. The principles illustrated here are also useful to all enzymatic quantitative methods. (C) 2001 Academic Press.

引用

页码：141 / 150

页数：10

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