In vitro malignant transformation of human bronchial epithelial cells induced by benzo(a)pyrene

被引：21

作者：

Zhao, Peng ^{[1
,2
]}

Fu, Juanling ^{[1
]}

Yao, Biyun ^{[1
,2
]}

Song, Yanchao ^{[1
,2
]}

Mi, Lan ^{[1
,2
]}

Li, Zhenning ^{[1
,2
]}

Shang, Lanqin ^{[1
,2
]}

Hao, Weidong ^{[1
,2
]}

Zhou, Zongcan ^{[1
]}

机构：

[1] Peking Univ, Hlth Sci Ctr, Dept Toxicol, Beijing 100191, Peoples R China

[2] Peking Univ, Hlth Sci Ctr, Beijing Key Lab Toxicol Res & Risk Assessment Foo, Beijing 100191, Peoples R China

来源：

TOXICOLOGY IN VITRO | 2012年 / 26卷 / 02期

关键词：

Benzo(a)pyrene; 16HBE cells; Malignant transformation; Cell migration; p53; p-Akt (Ser473); LUNG-CANCER; PROTEOMIC ANALYSIS; SIGNALING PATHWAY; EXPRESSION; G0/G1;

D O I：

10.1016/j.tiv.2011.12.013

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

In this study, the human bronchial epithelial cells (16HBE) were treated five times with 10 mu M benzo(a)pyrene (BaP), followed by 20 passages culture, and the in vitro BaP-induced malignant transformation of 16HBE cells was established. Five colonies in soft agarose were then amplified and donated as T-16HBE-C1 similar to 5 cells, respectively. T-16HBE-C1 similar to 5 cells can form tumors subcutaneously in nude mice. Histopathological changes in the tumors indicated nests growth, high nuclear-cytoplasmic ratios, coarse and clumped chromatin, numerous and distinctly atypical mitoses, cell necrosis and surrounding normal adipose, muscle and connective tissue immersed. In addition, lung metastasis was observed in nude mice in T-16HBE-C1, 3 and 4 groups. In vitro cell migration assay results indicated that T-16HBE-C2 similar to 5 cells showed much lower migration capabilities than 16HBE cells. Western blotting analysis showed that the expressions of p53 and p-Akt (Ser473) in T-16HBE-C1 similar to 5 cells were significant higher than those in 16HBE cells. Our results demonstrated that BaP could induce the malignant transformation of 16HBE cells, and p53 and p-Akt (Ser473) might play crucial roles in BaP-induced carcinogenesis. The five monoclonal cell lines (T-16HBE-C1 similar to 5) with different migration capabilities could be used as research models for further understanding the mechanisms of BaP-induced carcinogenesis and cell migration. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：362 / 368

页数：7

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