Selective human serum albumin sensor from the screening of a fluorescent rosamine library

被引：53
作者：
Ahn, Young-Hoon [1 ]
Lee, Jun-Seok [1 ,2 ,3 ]
Chang, Young-Tae [1 ,2 ,3 ,4 ]
机构：
[1] NYU, Dept Chem, New York, NY 10003 USA
[2] Natl Univ Singapore, Dept Chem, Singapore 117543, Singapore
[3] Natl Univ Singapore, NUS MedChem Program, Off Life Sci, Singapore 117543, Singapore
[4] ASTAR, Singapore Bioimaging Consortium, Lab Bioimaging Probe Dev, Biopolis 138667, Singapore
来源：
JOURNAL OF COMBINATORIAL CHEMISTRY | 2008年 / 10卷 / 03期
关键词：
D O I：
10.1021/cc800017h
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A fluorescent dye library approach for the development of a bioanalyte sensor was sought. The screening of a rosamine dye library against diverse macromolecules led to the discovery of a highly sensitive human serum albumin binder, G13, with similar to 36-fold fluorescence intensity change. G13 showed a highly selective response to HSA over other macromolecules including albumins from other species. The potential use of G13 for the detection of HSA in biofluids is described.
引用
收藏
页码：376 / 380
页数：5
相关论文
共 27 条
  • [1] Combinatorial rosamine library and application to in vivo glutathione probe
    Ahn, Young-Hoon
    Lee, Jun-Seok
    Chang, Young-Tae
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (15) : 4510 - +
  • [2] Solvent effect on absolute fluorescence quantum yield of rhodamine 6G determined using transient thermal lens technique
    Bindhu, CV
    Harilal, SS
    Nampoori, VPN
    Vallabhan, CPG
    [J]. MODERN PHYSICS LETTERS B, 1999, 13 (16): : 563 - 576
  • [3] Fluorescent sensors for Zn2+ based on a fluorescein platform:: Synthesis, properties and intracellular distribution
    Burdette, SC
    Walkup, GK
    Spingler, B
    Tsien, RY
    Lippard, SJ
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (32) : 7831 - 7841
  • [4] Crystal structure of human serum albumin complexed with fatty acid reveals an asymmetric distribution of binding sites
    Curry, S
    Mandelkow, H
    Brick, P
    Franks, N
    [J]. NATURE STRUCTURAL BIOLOGY, 1998, 5 (09) : 827 - 835
  • [5] Signaling recognition events with fluorescent sensors and switches
    de Silva, AP
    Gunaratne, HQN
    Gunnlaugsson, T
    Huxley, AJM
    McCoy, CP
    Rademacher, JT
    Rice, TE
    [J]. CHEMICAL REVIEWS, 1997, 97 (05) : 1515 - 1566
  • [6] ROLE OF ALBUMIN IN HUMAN PHYSIOLOGY AND PATHOPHYSIOLOGY
    DOWEIKO, JP
    NOMPLEGGI, DJ
    [J]. JOURNAL OF PARENTERAL AND ENTERAL NUTRITION, 1991, 15 (02) : 207 - 211
  • [7] A GENERAL, WIDE-RANGE SPECTROFLUOROMETRIC METHOD FOR MEASURING THE SITE-SPECIFIC AFFINITIES OF DRUGS TOWARD HUMAN SERUM-ALBUMIN
    EPPS, DE
    RAUB, TJ
    KEZDY, FJ
    [J]. ANALYTICAL BIOCHEMISTRY, 1995, 227 (02) : 342 - 350
  • [8] The binding of novel two-color fluorescence probe FA to serum albumins of different species
    Ercelen, S
    Klymchenko, AS
    Mély, Y
    Demchenko, AP
    [J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2005, 35 (05) : 231 - 242
  • [9] Interactions of lactone, carboxylate and self-aggregated forms of camptothecin with human and bovine serum albumins
    Fleury, F
    Kudelina, I
    Nabiev, I
    [J]. FEBS LETTERS, 1997, 406 (1-2) : 151 - 156
  • [10] Structure, dynamics, and stability variation in bacterial albumin binding modules: Implications for species specificity
    He, Yanan
    Rozak, David A.
    Sari, Nese
    Chen, Yihong
    Bryan, Philip
    Orban, John
    [J]. BIOCHEMISTRY, 2006, 45 (33) : 10102 - 10109
123