Constitutive expression of Brassica juncea annexin, AnnBj2 confers salt tolerance and glucose and ABA insensitivity in mustard transgenic plants

被引：23
作者：
Ahmed, Israr [1 ]
Yadav, Deepanker [1 ,4 ]
Shukla, Pawan [1 ,3 ]
Vineeth, T. V. [2 ]
Sharma, P. C. [2 ]
Kirti, P. B. [1 ]
机构：
[1] Univ Hyderabad, Dept Plant Sci, Hyderabad, Andhra Pradesh, India
[2] Cent Soil Salin Res Inst, Karnal, Haryana, India
[3] Cent Silk Board, Cent Sericultural Res & Training Inst, NH-1A, Pampore 192121, J&K, India
[4] Agr Res Org, Volcani Ctr, Inst Plant Sci, Dept Fruit Tree Sci, IL-7505101 Rishon Leziyyon, Israel
来源：
PLANT SCIENCE | 2017年 / 265卷
关键词：
ABA insensitive; ABI4; Annexin; Brassica juncea; Glucose insensitive; Salt tolerance; MEDIATED OXIDATIVE STRESS; BARLEY HORDEUM-VULGARE; K+-PERMEABLE CHANNELS; PLASMA-MEMBRANE CA2+; ABSCISIC-ACID; ARABIDOPSIS-THALIANA; SALINITY TOLERANCE; GENE FAMILY; HYDROXYL RADICALS; ENHANCES DROUGHT;
D O I：
10.1016/j.plantsci.2017.09.010
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Annexins belong to a plasma membrane binding (in a calcium dependent manner), multi-gene family of proteins, which play ameliorating roles in biotic and abiotic stresses. The expression of annexin AnnBj2 of Indian mustard is tissue specific with higher expression in roots and under treatments with sodium chloride and abscisic acid (ABA) at seedling stage. The effect of constitutive expression of AnnBj2 in mustard was analyzed in detail. AnnBj2 OE (over expression) plants exhibited insensitivity to ABA, glucose and sodium chloride. The insensitivity/tolerance of the transgenic plants was associated with enhanced total chlorophylls, relative water content, proline, calcium and potassium with reduced thiobarbituric acid reactive substances and sodium ion accumulation. The altered ABA insensitivity of AnnBj2 OE lines is linked to downregulation of ABI4 and ABI5 transcription factors and upregulation of ABA catabolic gene CYP707A2. Furthermore, we found that over expression of AnnBj2 upregulated the expression of ABA-dependent RAB18 and ABA-independent DREB2B stress marker genes suggesting that the tolerance phenotype exhibited by AnnBj2 OE lines is probably controlled by both ABA-dependent and - independent mechanisms.
引用
收藏
页码：12 / 28
页数：17
相关论文
共 121 条
[11]   Regulation and role of the Arabidopsis Abscisic Acid-Insensitive 5 gene in abscisic acid, sugar, and stress response [J].
Brocard, IM ;
Lynch, TJ ;
Finkelstein, RR .
PLANT PHYSIOLOGY, 2002, 129 (04) :1533-1543
[12]   Expression profiling of the Arabidopsis annexin gene family during germination, de-etiolation and abiotic stress [J].
Cantero, A ;
Barthakur, S ;
Bushart, TJ ;
Chou, S ;
Morgan, RO ;
Fernandez, MP ;
Clark, GB ;
Roux, SJ .
PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2006, 44 (01) :13-24
[13]   Difference in root K+ retention ability and reduced sensitivity of K+-permeable channels to reactive oxygen species confer differential salt tolerance in three Brassica species [J].
Chakraborty, Koushik ;
Bose, Jayakumar ;
Shabala, Lana ;
Shabala, Sergey .
JOURNAL OF EXPERIMENTAL BOTANY, 2016, 67 (15) :4611-4625
[14]   Biological roles of cyclic-nucleotide-gated ion channels in plants: What we know and don't know about this 20 member ion channel family [J].
Chin, Kimberley ;
Moeder, Wolfgang ;
Yoshioka, Keiko .
BOTANY, 2009, 87 (07) :668-677
[15]   Proteomic and functional analyses of Nelumbo nucifera annexins involved in seed thermotolerance and germination vigor [J].
Chu, Pu ;
Chen, Huhui ;
Zhou, Yuliang ;
Li, Yin ;
Ding, Yu ;
Jiang, Liwen ;
Tsang, Edward W. T. ;
Wu, Keqiang ;
Huang, Shangzhi .
PLANTA, 2012, 235 (06) :1271-1288
[16]   Differential expression of members of the annexin multigene family in Arabidopsis [J].
Clark, GB ;
Sessions, A ;
Eastburn, DJ ;
Roux, SJ .
PLANT PHYSIOLOGY, 2001, 126 (03) :1072-1084
[17]   Evolutionary adaptation of plant annexins has diversified their molecular structures, interactions and functional roles [J].
Clark, Greg B. ;
Morgan, Reginald O. ;
Fernandez, Maria-Pilar ;
Roux, Stanley J. .
NEW PHYTOLOGIST, 2012, 196 (03) :695-712
[18]   DISPLACEMENT OF CA-2+ BY NA-+ FROM THE PLASMALEMMA OF ROOT-CELLS - A PRIMARY RESPONSE TO SALT STRESS [J].
CRAMER, GR ;
LAUCHLI, A ;
POLITO, VS .
PLANT PHYSIOLOGY, 1985, 79 (01) :207-211
[19]   A root's ability to retain K+ correlates with salt tolerance in wheat [J].
Cuin, Tracey Ann ;
Betts, Stewart A. ;
Chalmandrier, Remi ;
Shabala, Sergey .
JOURNAL OF EXPERIMENTAL BOTANY, 2008, 59 (10) :2697-2706
[20]   Abscisic Acid: Emergence of a Core Signaling Network [J].
Cutler, Sean R. ;
Rodriguez, Pedro L. ;
Finkelstein, Ruth R. ;
Abrams, Suzanne R. .
ANNUAL REVIEW OF PLANT BIOLOGY, VOL 61, 2010, 61 :651-679
12345678910