Physical, chemical and kinetic factors affecting prion infectivity

被引：2

作者：

Properzi, Francesca ^{[1
]}

Badhan, Anjna ^{[1
]}

Klier, Steffi ^{[1
]}

Schmidt, Christian ^{[1
]}

Klohn, Peter C. ^{[1
]}

Wadsworth, Jonathan D. F. ^{[1
]}

Clarke, Anthony R. ^{[1
]}

Jackson, Graham S. ^{[1
]}

Collinge, John ^{[1
]}

机构：

[1] UCL Inst Neurol, Dept Neurodegenerat Dis, MRC Pr Unit, London, England

来源：

PRION | 2016年 / 10卷 / 03期

基金：

英国医学研究理事会;

关键词：

BSE; CJD; cell culture; prion; PrP; RML; scrapie; INTERMOLECULAR DISULFIDE BONDS; IN-VITRO; PROTEIN; SCRAPIE; PARTICLES; RESISTANT; DISEASES;

D O I：

10.1080/19336896.2016.1181250

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mouse-adapted scrapie prion strain RML is one of the most widely used in prion research. The introduction of a cell culture-based assay of RML prions, the scrapie cell assay (SCA) allows more rapid and precise prion titration. A semi-automated version of this assay (ASCA) was applied to explore a range of conditions that might influence the infectivity and properties of RML prions. These include resistance to freeze-thaw procedures; stability to endogenous proteases in brain homogenate despite prolonged exposure to varying temperatures; distribution of infective material between pellet and supernatant after centrifugation, the effect of reducing agents and the influence of detergent additives on the efficiency of infection. Apparent infectivity is increased significantly by interaction with cationic detergents. Importantly, we have also elucidated the relationship between the duration of exposure of cells to RML prions and the transmission of infection. We established that the infection process following contact of cells with RML prions is rapid and followed an exponential time course, implying a single rate-limiting process.

引用

页码：251 / 261

页数：11

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