Global changes in and characterization of specific sites of phosphorylation in mouse and human histone H1 isoforms upon CDK inhibitor treatment using mass spectrometry

被引：23

作者：

Deterding, Leesa J. ^{[1
]}

Bunger, Maureen K. ^{[2
]}

Banks, Geoffrey C. ^{[2
]}

Tomer, Kenneth B. ^{[1
]}

Archer, Trevor K. ^{[2
]}

机构：

[1] NIEHS, Struct Biol Lab, NIH, DHHS, Res Triangle Pk, NC 27709 USA

[2] NIEHS, Mol Carcinogenesis Lab, NIH, DHHS, Res Triangle Pk, NC 27709 USA

来源：

JOURNAL OF PROTEOME RESEARCH | 2008年 / 7卷 / 06期

关键词：

H1; histories; chromatin; phosphorylation; mass spectrometry; CDK inhibitors; tandem mass spectrometry;

D O I：

10.1021/pr700790a

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Global changes in the phosphorylation state of human H1 isoforms isolated from UL3 cells have been investigated using mass spectrometry. Relative changes in H1 phosphorylation between untreated cells and cells treated with dexamethasone or various CDK inhibitors were determined. The specific cyclin-dependent kinase consensus sites of phosphorylation on the histone H1 isoforms that show changes in phosphorylation were also investigated. Three sites of phosphorylation on histone H1.4 isoforms have been identified.

引用

页码：2368 / 2379

页数：12

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