Control of Specific/Nonspecific Protein Adsorption: Functionalization of Polyelectrolyte Multilayer Films as a Potential Coating for Biosensors

被引:9
作者
Kruk, Tomasz [1 ]
Bzowska, Monika [2 ]
Hinz, Alicja [2 ]
Szuwarzynski, Michal [3 ]
Szczepanowicz, Krzysztof [1 ]
机构
[1] Polish Acad Sci, Jerzy Haber Inst Catalysis & Surface Chem, Niezapominajek 8, PL-30239 Krakow, Poland
[2] Jagiellonian Univ, Fac Biochem Biophys & Biotechnol, Dept Cell Biochem, PL-30348 Krakow, Poland
[3] AGH Univ Sci & Technol, Acad Ctr Mat & Nanotechnol, Mickiewicza 30, PL-30059 Krakow, Poland
关键词
polyelectrolyte multilayers; poly (ethylene glycol); protein-resistant surfaces; protein adsorption; specific adsorption; POLY(L-LYSINE)-G-POLY(ETHYLENE GLYCOL) LAYERS; SELF-ASSEMBLY PROCESS; METAL-OXIDE SURFACES; IN-VITRO; RESISTANCE; ARCHITECTURE; MONOLAYERS; BUILDUP; SERUM;
D O I
10.3390/ma14247629
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Control of nonspecific/specific protein adsorption is the main goal in the design of novel biomaterials, implants, drug delivery systems, and sensors. The specific functionalization of biomaterials can be achieved by proper surface modification. One of the important strategies is covering the materials with functional coatings. Therefore, our work aimed to functionalize multilayer coating to control nonspecific/specific protein adsorption. The polyelectrolyte coating was formed using a layer-by-layer technique (LbL) with biocompatible polyelectrolytes poly-L-lysine hydrobromide (PLL) and poly-L-glutamic acid (PGA). Nonspecific protein adsorption was minimized/eliminated by pegylation of multilayer films, which was achieved by adsorption of pegylated polycations (PLL-g-PEG). The influence of poly (ethylene glycol) chain length on eliminating nonspecific protein adsorption was confirmed. Moreover, to achieve specific protein adsorption, the multilayer film was also functionalized by immobilization of antibodies via a streptavidin bridge. The functional coatings were tested, and the adsorption of the following proteins confirmed the ability to control nonspecific/specific adsorption: human serum albumin (HSA), fibrinogen (FIB), fetal bovine serum (FBS), carcinoembryonic antigen human (CEA) monitored by quartz crystal microbalance with dissipation (QCM-D). AFM imaging of unmodified and modified multilayer surfaces was also performed. Functional multilayer films are believed to have the potential as a novel platform for biotechnological applications, such as biosensors and nanocarriers for drug delivery systems.
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页数:13
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