A novel homogenous detection method based on the self-assembled DNAzyme labeled DNA probes with SWNT conjugates and its application in detecting pathogen

被引:19
作者
Ding, Xinghua
Li, Hua
Deng, Le [1 ]
Peng, Zhihui
Chen, Hui
Wang, Dan
机构
[1] Hunan Normal Univ, Coll Life Sci, Dept Microbiol, Minist Sci & Technol, Changsha 410081, Hunan, Peoples R China
关键词
Homogeneous detection method; DNAzyme; Self-assembly; SWNTs; Protection; Pathogen detection; CARBON NANOTUBES; SENSING PLATFORM; SENSORS;
D O I
10.1016/j.bios.2011.04.041
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
In this paper, a novel and cost-effective homogeneous detection method was constructed for the detection of genomic DNA and Staphylococcus aureus (S. aureus), based on the noncovalent assembly of DNAzyme-labeled detection probe and single-walled carbon nanotubes (SWNTs). When the target genomic DNA and hemin was existed in the detection solution, the detection probe wrapped on the SWNTs by pi-stacking interactions would keep away from SWNTs and form a DNAzyme-self-assembly construction. This DNAzyme construction could catalyze 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS(2-)) and generate a colored product which could lead to the absorbance changes. Hence, according to its catalyzed capacity, the DNAzyme construction could amplify the detection signal. The concentration of target DNA could be quantified by exploiting their optical absorption changes at 414 nm and the concentration limit of detection of the method was 30 nM. And this detection method detected S. aureus quantitatively. In addition, this work proved that the method obtain higher detection sensitivity compared with the method without SWNTs because of the protection profile of SWNTs towards the detection probe. Crown Copyright (C) 2011 Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:4596 / 4600
页数:5
相关论文
共 25 条
  • [1] Nanotubes from carbon
    Ajayan, PM
    [J]. CHEMICAL REVIEWS, 1999, 99 (07) : 1787 - 1799
  • [2] Nano-biosensor development for bacterial detection during human kidney infection: Use of glycoconjugate-specific antibody-bound gold NanoWire arrays (GNWA)
    Basu, M
    Seggerson, S
    Henshaw, J
    Jiang, J
    Cordona, RD
    Lefave, C
    Boyle, PJ
    Miller, A
    Pugia, M
    Basu, S
    [J]. GLYCOCONJUGATE JOURNAL, 2004, 21 (8-9) : 487 - 496
  • [3] Briza P.L., 2009, ANALYST, V134, P60
  • [4] Enzyme-amplified amperometric sandwich test for RNA and DNA
    Campbell, CN
    Gal, D
    Cristler, N
    Banditrat, C
    Heller, A
    [J]. ANALYTICAL CHEMISTRY, 2002, 74 (01) : 158 - 162
  • [5] Di L., 2007, J AM CHEM SOC, V129, P5804
  • [6] Electrochemical DNA sensors
    Drummond, TG
    Hill, MG
    Barton, JK
    [J]. NATURE BIOTECHNOLOGY, 2003, 21 (10) : 1192 - 1199
  • [7] Ordering fullerene materials at manometer dimensions
    Guldi, DM
    Zerbetto, F
    Georgakilas, V
    Prato, M
    [J]. ACCOUNTS OF CHEMICAL RESEARCH, 2005, 38 (01) : 38 - 43
  • [8] Use of different stains for microscopic evaluation of corneal scrapings for diagnosis of microsporidial keratitis
    Joseph, J
    Murthy, S
    Garg, P
    Sharma, S
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (02) : 583 - 585
  • [9] Nanotube molecular transporters: Internalization of carbon nanotube-protein conjugates into mammalian cells
    Kam, NWS
    Jessop, TC
    Wender, PA
    Dai, HJ
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (22) : 6850 - 6851
  • [10] Fluorescent assay of DNA hybridization with label-free molecular switch: reducing background-signal and improving specificity by using carbon nanotubes
    Liu, Yue
    Wang, Yongxiang
    Jin, Jianyu
    Wang, Hao
    Yang, Ronghua
    Tan, Weihong
    [J]. CHEMICAL COMMUNICATIONS, 2009, (06) : 665 - 667