Overexpression of OsTF1L, a rice HD-Zip transcription factor, promotes lignin biosynthesis and stomatal closure that improves drought tolerance

被引：106

作者：

Bang, Seung Woon ^{[1
,2
,3
]}

Lee, Dong-Keun ^{[1
,2
]}

Jung, Harin ^{[1
,2
,5
]}

Chung, Pil Joong ^{[1
,2
]}

Kim, Youn Shic ^{[1
,2
]}

Choi, Yang Do ^{[4
]}

Suh, Joo-Won ^{[3
]}

Kim, Ju-Kon ^{[1
,2
]}

机构：

[1] Seoul Natl Univ, Grad Sch Int Agr Technol, Pyeongchang, South Korea

[2] Seoul Natl Univ, Crop Biotechnol Inst, GreenBio Sci & Technol, Pyeongchang, South Korea

[3] Myongji Univ, Div Bioinformat, Ctr Nutraceut & Pharmaceut Mat, Yongin, Gyeonggi, South Korea

[4] Seoul Natl Univ, Dept Agr Biotechnol, Seoul, South Korea

[5] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Biochem, NUS Synthet Biol Clin & Technol Innovat, Singapore 117596, Singapore

来源：

PLANT BIOTECHNOLOGY JOURNAL | 2019年 / 17卷 / 01期

基金：

新加坡国家研究基金会;

关键词：

drought tolerance; Oryza sativa; OsTF1L; lignin biosynthesis; stomatal closure; HD-Zip transcription factor; GL2-TYPE HOMEOBOX GENE; TRANSGENIC RICE; SALT TOLERANCE; GRAIN-YIELD; STRESS TOLERANCE; EMBOLISM RESISTANCE; ROOT ELONGATION; CANDIDATE GENES; WALL FORMATION; ORYZA-SATIVA;

D O I：

10.1111/pbi.12951

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Drought stress seriously impacts on plant development and productivity. Improvement of drought tolerance without yield penalty is a great challenge in crop biotechnology. Here, we report that the rice (Oryza sativa) homeodomain-leucine zipper transcription factor gene, OsTF1L (Oryza sativa transcription factor 1-like), is a key regulator of drought tolerance mechanisms. Overexpression of the OsTF1L in rice significantly increased drought tolerance at the vegetative stages of growth and promoted both effective photosynthesis and a reduction in the water loss rate under drought conditions. Importantly, the OsTF1L overexpressing plants showed a higher drought tolerance at the reproductive stage of growth with a higher grain yield than nontransgenic controls under field-drought conditions. Genomewide analysis of OsTF1L overexpression plants revealed up-regulation of drought-inducible, stomatal movement and lignin biosynthetic genes. Overexpression of OsTF1L promoted accumulation of lignin in shoots, whereas the RNAi lines showed opposite patterns of lignin accumulation. OsTF1L is mainly expressed in outer cell layers including the epidermis, and the vasculature of the shoots, which coincides with areas of lignification. In addition, OsTF1L overexpression enhances stomatal closure under drought conditions resulted in drought tolerance. More importantly, OsTF1L directly bound to the promoters of lignin biosynthesis and drought-related genes involving poxN/PRX38, Nodulin protein, DHHC4, CASPL5B1 and AAA-type ATPase. Collectively, our results provide a new insight into the role of OsTF1L in enhancing drought tolerance through lignin biosynthesis and stomatal closure in rice.

引用

页码：118 / 131

页数：14