Pseudoruegeria insulae sp nov., isolated from a tidal flat

A Gram-stain-negative, aerobic, non-motile and ovoid or rod-shaped bacterial strain, designated BPTF-M20(T), was isolated from tidal flat sediment in the Yellow Sea, Republic of Korea. Strain BPTF-M20(T) grew optimally at 30 degrees C, at pH 7.0-8.0 and in the presence of 2.0-3.0% (w/v) NaCl. A neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain BPTF-M20(T) fell within the clade comprising the type strains of Pseudoruegeria species. Strain BPTF-M20(T) exhibited 16S rRNA gene sequence similarity values of 97.4-98.3% to the type strains of Pseudoruegeria haliotis, Pseudoruegeria lutimaris, 'seudoruegeria litorisediminis' and Pseudoruegeria sabulilitoris and 96.4-96.9% to the type strains of the other Pseudoruegeria species. Strain BPTF-M20(T) contained Q-10 as the predominant ubiquinone and C-18:(1)omega 7c as the major fatty acid. The major polar lipids detected in strain BPTF-M20(T) were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminolipid and one unidentified glycolipid. The DNA G+C content of strain BPTF-M20(T) was 63.2 mol%. Mean DNA-DNA relatedness values of strain BPTF-M20(T) with the type strains of P. haliotis, P. lutimaris, P. sabulilitoris and 'P. litorisediminis' were 18-27 %. Differential phenotypic properties, together with the phylogenetic and genetic data, revealed that strain BPTF-M20(T) was separated from recognized Pseudoruegeria species. On the basis of the data presented here, strain BPTF-M20(T) is considered to represent a novel species of the genus Pseudoruegeria, for which the name Pseudoruegeria insulae sp. nov. is proposed. The type strain is BPTF-M20(T) (=KACC 19614(T) =KCTC 62422(T) =NBRC 113188(T)).