Fe3O4 nanoparticles-enhanced SPR sensing for ultrasensitive sandwich bio-assay

被引：75
作者：
Wang, Jianlong [1 ]
Zhu, Zanzan [1 ]
Munir, Ahsan [1 ]
Zhou, H. Susan [1 ]
机构：
[1] Worcester Polytech Inst, Dept Chem Engn, Worcester, MA 01609 USA
来源：
TALANTA | 2011年 / 84卷 / 03期
基金：
美国国家科学基金会;
关键词：
Magnetic nanoparticles; Surface plasmon resonance; Sandwich bio-assay; SURFACE-PLASMON RESONANCE; IRON-OXIDE NANOCRYSTALS; MAGNETIC NANOPARTICLES; SMALL MOLECULES; ELECTROCHEMICAL DETECTION; SIGNAL AMPLIFICATION; DNA HYBRIDIZATION; ALPHA-FETOPROTEIN; APTAMER; SENSOR;
D O I：
10.1016/j.talanta.2011.02.020
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Magnetic nanoparticles (MNPs) have been receiving increasing attention because of its great potentials in bioseparation. However, the separation products are difficult to be detected by general method due to their extremely small size. Here, we demonstrate that MNPs can greatly enhance the signal of surface plasmon resonance spectroscopy (SPR). Features of MNPs-aptamer conjugates as a powerful amplification reagent for ultrasensitive immunoassay are reported in this work for the first time. In order to evaluate the sensing ability of MNPs-aptamer conjugates as an amplification reagent, a sandwich SPR sensor is constructed by using thrombin as model analyte. Thrombin, captured by immobilized antithrombin aptamer on SPR gold film, is sensitively detected by SPR spectroscopy with a lowest detection limit of 0.017 nM after MNPs-aptamer conjugates is used as amplification reagent. At the same time, the excellent selectivity of the present biosensor is also confirmed by using three kinds of proteins (BSA, human IgM and human IgE) as controls. These results confirm that MNPs is a powerful sandwich element and an excellent amplification reagent for SPR based sandwich immunoassay and SPR has a great potential for the detection of MNPs-based bioseparation products. Published by Elsevier B.V.
引用
收藏
页码：783 / 788
页数：6
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