Foxa1 mediates eccrine sweat gland development through transcriptional regulation of Na-K-ATPase expression

被引：0

作者：

Zhao, Junhong ^{[1
]}

Zhang, Lei ^{[2
]}

Du, Lijie ^{[1
]}

Chen, Zixiu ^{[1
]}

Tang, Yue ^{[1
]}

Chen, Lijun ^{[1
]}

Liu, Xiang ^{[1
]}

You, Lei ^{[3
]}

Zhang, Yonghong ^{[3
]}

Fu, Xiaobing ^{[4
]}

Li, Haihong ^{[1
,5
,6
]}

机构：

[1] Hubei Univ Med, Taihe Hosp, Dept Wound Repair & Dermatol Surg, Shiyan, Hubei, Peoples R China

[2] Hubei Univ Med, Taihe Hosp, Mental Hlth Ctr, Shiyan, Hubei, Peoples R China

[3] Hubei Univ Med, Acad Biomed Res, Sch Basic Med, Shiyan, Hubei, Peoples R China

[4] Chinese Peoples Liberat Army Gen Hosp, Affiliated Hosp 1, Wound Healing & Cell Biol Lab, Beijing, Peoples R China

[5] Shantou Univ, Affiliated Hosp 2, Med Coll, Dept Plast Surg, Shantou, Guangdong, Peoples R China

[6] Shantou Univ, Affiliated Hosp 2, Med Coll, Burn Ctr, Shantou, Guangdong, Peoples R China

来源：

BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH | 2022年 / 55卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Forkhead box A1; Na-K-ATPase; Eccrine sweat glands; BASOLATERAL PLASMA-MEMBRANE; NA+-K+-2CL(-) COTRANSPORTER; RAT; CANCER; CELLS;

D O I：

10.1590/1414-431X2022e12149

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Eccrine sweat glands (ESGs) perform critical functions in temperature regulation in humans. Foxa1 plays an important role in ESG maturation and sweat secretion. Its molecular mechanism, however, remains unknown. This study investigated the expression of Foxa1 and Na-K-ATPase (NKA) in rat footpads at different development stages using immunofluorescence staining, qRT-PCR, and immunoblotting. Also, bioinformatics analysis and Foxa1 overexpression and silencing were employed to evaluate Foxa1 regulation of NKA. The results demonstrated that Foxa1 was consistently expressed during the late stages of ESGs and had a significant role in secretory coil maturation during sweat secretion. Furthermore, the mRNA abundance and protein expression of NKA had similar accumulation trends to those of Foxa1, confirming their underlying connections. Bioinformatics analysis revealed that Foxa1 may interact with these two proteins via binding to conserved motifs in their promoter regions. Foxa1 gain-of-function and loss-of-function experiments in Foxa1-modified cells demonstrated that the activities of NKA were dependent on the presence of Foxa1. Collectively, these data provided evidence that Foxa1 may influence ESG development through transcriptional regulation of NKA expression.

引用

页数：10

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