Development of a Xeno-Free Substrate for Human Embryonic Stem Cell Growth

被引：4
作者：
Zhu, Hailin [1 ,2 ]
Yang, Jinliang [1 ]
Wei, Yuquan [1 ]
Chen, Harry Huimin [1 ,2 ]
机构：
[1] Sichuan Univ, State Key Lab Biotherapy, Chengdu 610041, Sichuan, Peoples R China
[2] StemEasy Biotech Ltd, Jiang Yin 214400, Jiangsu, Peoples R China
来源：
STEM CELLS INTERNATIONAL | 2015年 / 2015卷
关键词：
PROLONGED UNDIFFERENTIATED GROWTH; RECOMBINANT VITRONECTIN; HUMAN FEEDERS; SELF-RENEWAL; DERIVATION; LINES;
D O I：
10.1155/2015/621057
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Traditionally, human embryonic stem cells (hESCs) are cultured on inactivated live feeder cells. For clinical application using hESCs, there is a requirement to minimize the risk of contamination with animal components. Extracellular matrix (ECM) derived from feeder cells is the most natural way to provide xeno-free substrates for hESC growth. In this study, we optimized the step-by-step procedure for ECM processing to develop a xeno-free ECM that supports the growth of undifferentiated hESCs. In addition, this newly developed xeno-free substrate can be stored at 4 degrees C and is ready to use upon request, which serves as an easier way to amplify hESCs for clinical applications.
引用
收藏
页数：8
相关论文
共 50 条
  • [31] Xeno-Free and Defined Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells Functionally Integrate in a Large-Eyed Preclinical Model
    Reyes, Alvaro Plaza
    Petrus-Reurer, Sandra
    Antonsson, Liselotte
    Stenfelt, Sonya
    Bartuma, Hammurabi
    Panula, Sarita
    Mader, Theresa
    Douagi, Iyadh
    Andre, Helder
    Hovatta, Outi
    Lanner, Fredrik
    Kvanta, Anders
    STEM CELL REPORTS, 2016, 6 (01): : 9 - 17
  • [32] Development of a simple, repeatable, and cost-effective extracellular matrix for long-term xeno-free and feeder-free self-renewal of human pluripotent stem cells
    Pakzad, Mohammad
    Ashtiani, Mohammad Kazemi
    Mousavi-Gargari, Seyed Latif
    Baharvand, Hossein
    HISTOCHEMISTRY AND CELL BIOLOGY, 2013, 140 (06) : 635 - 648
  • [33] Xeno-free culture of human pluripotent stem cells on oligopeptide-grafted hydrogels with various molecular designs
    Chen, Yen-Ming
    Chen, Li-Hua
    Li, Meng-Pei
    Li, Hsing-Fen
    Higuchi, Akon
    Kumar, S. Suresh
    Ling, Qing-Dong
    Alarfaj, Abdullah A.
    Munusamy, Murugan A.
    Chang, Yung
    Benelli, Giovanni
    Murugan, Kadarkarai
    Umezawa, Akihiro
    SCIENTIFIC REPORTS, 2017, 7
  • [34] Defined and Serum-Free Media Support Undifferentiated Human Embryonic Stem Cell Growth
    Chin, Angela Chui Ping
    Padmanabhan, Jayanthi
    Oh, Steve Kah Weng
    Choo, Andre Boon Hwa
    STEM CELLS AND DEVELOPMENT, 2010, 19 (06) : 753 - 761
  • [35] Design of polymeric materials for culturing human pluripotent stem cells: Progress toward feeder-free and xeno-free culturing
    Higuchi, Akon
    Ling, Qing-Dong
    Kumar, Suresh
    Munusamy, Murugan
    Alarfajj, Abdullah A.
    Umezawa, Akihiro
    Wu, Gwo-Jang
    PROGRESS IN POLYMER SCIENCE, 2014, 39 (07) : 1348 - 1374
  • [36] A xeno-free culturing protocol for pluripotent stem cell-derived oligodendrocyte precursor cell production
    Sundberg, Maria
    Hyysalo, Anu
    Skottman, Heli
    Shin, Soojung
    Vemuri, Mohan
    Suuronen, Riitta
    Narkilahti, Susanna
    REGENERATIVE MEDICINE, 2011, 6 (04) : 449 - 460
  • [37] Development of a Xeno-Free Feeder-Layer System from Human Umbilical Cord Mesenchymal Stem Cells for Prolonged Expansion of Human Induced Pluripotent Stem Cells in Culture
    Zou, Qing
    Wu, Mingjun
    Zhong, Liwu
    Fan, Zhaoxin
    Zhang, Bo
    Chen, Qiang
    Ma, Feng
    PLOS ONE, 2016, 11 (02):
  • [38] An ECM-based culture system for the generation and maintenance of xeno-free human iPS cells
    Kim, Hyeong-Taek
    Lee, Kang-In
    Kim, Dong-Wook
    Hwang, Dong-Youn
    BIOMATERIALS, 2013, 34 (04) : 1041 - 1050
  • [39] Enhanced Expansion of Human Pluripotent Stem Cells and Somatic Cell Reprogramming Using Defined and Xeno-Free Culture Conditions
    Timilsina, Suraj
    McCandliss, Kaitlyn Faith
    Trivedi, Evan
    Villa-Diaz, Luis G.
    BIOENGINEERING-BASEL, 2023, 10 (09):
  • [40] Generation of clinical-grade human induced pluripotent stem cells in Xeno-free conditions
    Wang, Juan
    Hao, Jie
    Bai, Donghui
    Gu, Qi
    Han, Weifang
    Wang, Lei
    Tan, Yuanqing
    Li, Xia
    Xue, Ke
    Han, Pencheng
    Liu, Zhengxin
    Jia, Yundan
    Wu, Jun
    Liu, Lei
    Wang, Liu
    Li, Wei
    Liu, Zhonghua
    Zhou, Qi
    STEM CELL RESEARCH & THERAPY, 2015, 6
12345