Rapid determination of hazardous compounds in food based on a competitive fluorescence microsphere immunoassay

被引：24

作者：

Zou, Mingqlang ^{[1
]}

Gao, Haixia ^{[1
,2
]}

Li, Jinfeng ^{[1
]}

Xu, Fang ^{[3
]}

Wang, Ling ^{[1
,2
]}

Jiang, Jizhi ^{[2
]}

机构：

[1] Chinese Acad Inspect & Quarantine, Beijing 100025, Peoples R China

[2] Hebei Univ, Coll Life Sci, Baoding 071002, Peoples R China

[3] Jiao Tong Univ, Sch Environm Sci & Engn, Shanghai 200030, Peoples R China

来源：

ANALYTICAL BIOCHEMISTRY | 2008年 / 374卷 / 02期

基金：

中国国家自然科学基金;

关键词：

microsphere-based competitive fluorescence immunoassay; low-molecular-weight compounds; flow cytometry; microsphere; clenbuterol residue;

D O I：

10.1016/j.ab.2007.10.024

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Development of a microsphere-based competitive fluorescence immunoassay for the determination of hazardous low-molecular-weight compounds in food is described. In this method, antigens are covalently bound to carboxy-modified microspheres to compete monoclonal antibody with low-molecular-weight compounds in food samples; mouse IgG/fluorescein isothiocyanate conjugate is used as the fluorescent molecular probe. Thus, the hazardous low-molecular-weight compounds are quantified using a multiparameter flow cytometer. This method has been evaluated using clenbuterol as a model compound. It has a sensitivity of 0.01 ng/mL with dynamic range of 0.01-100ng/mL, and the concentration of clenbuterol providing 50% inhibition (IC50) is 1.1 ng/mL. The main advantages of this method are its high efficiency, biocompatibility, and selectivity, as well as ultralow trace sample consumption and low cost. (C) 2007 Elsevier Inc. All rights reserved.

引用

页码：318 / 324

页数：7

共 23 条

[1] A fiber optic biosensor for multianalyte detection: importance of preventing fluorophore aggregation [J].

Bakaltcheva, IB ;

Shriver-Lake, LC ;

Ligler, FS .

SENSORS AND ACTUATORS B-CHEMICAL, 1998, 51 (1-3) :46-51

[2] Method for simultaneous measurement of antibodies to 23 pneumococcal capsular polysaccharides [J].

Biagini, RE ;

Schlottmann, SA ;

Sammons, DL ;

Smith, JP ;

Snawder, JC ;

Striley, CAF ;

MacKenzie, BA ;

Weissman, DN .

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 2003, 10 (05) :744-750

[3] Prioritising veterinary medicines according to their potential indirect human exposure and toxicity profile [J].

Capleton, AC ;

Courage, C ;

Rumsby, P ;

Holmes, P ;

Stutt, E ;

Boxall, ABA ;

Levy, LS .

TOXICOLOGY LETTERS, 2006, 163 (03) :213-223

[4] Simultaneous quantitation of 15 cytokines using a multiplexed flow cytometric assay [J].

Carson, RT ;

Vignali, DAA .

JOURNAL OF IMMUNOLOGICAL METHODS, 1999, 227 (1-2) :41-52

[5] A comparison of ELISA and flow micro sphere-based assays for quantification of immunoglobulins [J].

Dasso, J ;

Lee, J ;

Bach, H ;

Mage, RG .

JOURNAL OF IMMUNOLOGICAL METHODS, 2002, 263 (1-2) :23-33

[6] Toxicological evaluation of chemical mixtures [J].

Feron, VJ ;

Groten, JP .

FOOD AND CHEMICAL TOXICOLOGY, 2002, 40 (06) :825-839

[7] Comparison of a commercial ELISA with the modified agglutination test for detection of Toxoplasma infection in the domestic pig [J].

Gamble, HR ;

Dubey, JP ;

Lambillotte, DN .

VETERINARY PARASITOLOGY, 2005, 128 (3-4) :177-181

[8] Health risk assessment practices in the US Food and Drug Administration [J].

Gaylor, DW ;

Axelrad, JA ;

Brown, RP ;

Cavagnaro, JA ;

Cyr, WH ;

Hulebak, KL ;

Lorentzen, RJ ;

Miller, MA ;

Mulligan, LT ;

Schwetz, BA .

REGULATORY TOXICOLOGY AND PHARMACOLOGY, 1997, 26 (03) :307-321

[9] LC-MS methods for analyzing anti-inflammatory drugs in animal-food products [J].

Gentili, Alessandra .

TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2007, 26 (06) :595-608

[10] THEORETICAL LIMITATIONS ON IMMUNOASSAY SENSITIVITY - CURRENT PRACTICE AND POTENTIAL ADVANTAGES OF FLUORESCENT EU-3+ CHELATES AS NONRADIOISOTOPIC TRACERS [J].

JACKSON, TM ;

EKINS, RP .

JOURNAL OF IMMUNOLOGICAL METHODS, 1986, 87 (01) :13-20

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