Comparative proteomic analysis of juvenile and adult liver fluke, Opisthorchis viverrini

被引：10
作者：
Boonmee, S [1 ]
Imtawil, K [1 ]
Wongkham, C [1 ]
Wongkham, S [1 ]
机构：
[1] Khon Kaen Univ, Fac Med, Dept Biochem, Khon Kaen 40002, Thailand
来源：
ACTA TROPICA | 2003年 / 88卷 / 03期
关键词：
Opisthorchis viverrini; proteomics; 2-DE; liver fluke;
D O I：
10.1016/j.actatropica.2003.09.006
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
We used comparative two-dimensional gel electrophoresis to highlight proteins that are differentially expressed in the maturation stage of the parasite Opisthorchis viverrini (OV). The proteins differentially expressed in the juvenile/adult forms of the parasite are thought to be important for survival and pathogenesis. We used a nonlinear gradient pH ranged 3-10 strips for isoelectric focusing to resolve soluble proteins from four different maturation periods of OV from 1 week juvenile to 4 week adult. Approximately 210-240 protein spots were resolved by 2-DE in two ranges of pI (4.5-5.8 and 6.0-8.0). At least 35 protein spots were differentially expressed in 4 week adult compared to 1 week juvenile fluke. These proteins may involve in sex organ development and egg production. Comparative analysis of the OV proteome of different aged parasites during maturation may help to better understand parasite biology, pathogenesis/carcinogenesis related to this parasite and lead to the identification of new targets of vaccines and drugs. (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码：233 / 238
页数：6
相关论文
共 50 条
[41]   Stage-specific expression and antigenicity of glycoprotein glycans isolated from the human liver fluke, Opisthorchis viverrini [J].
Talabnin, Krajang ;
Aoki, Kazuhiro ;
Saichua, Prasert ;
Wongkham, Sopit ;
Kaewkes, Sasithorn ;
Boons, Geert-Jan ;
Sripa, Banchob ;
Tiemeyer, Michael .
INTERNATIONAL JOURNAL FOR PARASITOLOGY, 2013, 43 (01) :37-50
[42]   Detection of the carcinogenic liver fluke, Opisthorchis viverrini: comparison of two coprological methods versus the automatic feces analyzer [J].
Boonsuya, A. ;
Arunsan, P. ;
Pechdee, P. ;
La, N. ;
Thanchonnang, C. ;
Rattanapitoon, N. K. ;
Rattanapitoon, S. K. .
TROPICAL BIOMEDICINE, 2024, 41 (03) :264-270
[43]   SURFACE DISPLAY ON BACILLUS SUBTILIS SPORES AND VACCINE POTENTIAL OF A TETRASPANIN FROM CARCINOGENIC LIVER FLUKE, OPISTHORCHIS VIVERRINI [J].
Phumrattanaprapin, Wuttipong ;
Chaiyadet, Sujittra ;
Loukas, Alex ;
Brindley, Paul J. ;
Sotillo, Javier ;
Laha, Thewarach .
SOUTHEAST ASIAN JOURNAL OF TROPICAL MEDICINE AND PUBLIC HEALTH, 2018, 49 (06) :933-948
[44]   Human liver fluke Opisthorchis viverrini (Trematoda, Opisthorchiidae) in Central Myanmar: New records of adults and metacercariae identified by morphology and molecular analysis [J].
Sanpool, Oranuch ;
Aung, Win Pa Pa ;
Rodpai, Rutchanee ;
Maleewong, Wanchai ;
Intapan, Pewpan M. .
ACTA TROPICA, 2018, 185 :149-155
[45]   Retrotransposon OV-RTE-1 from the carcinogenic liver fluke Opisthorchis viverrini: Potential target for DNA-based diagnosis [J].
Luyen Thi Phung ;
Loukas, Alex ;
Brindley, Paul J. ;
Sripa, Banchob ;
Laha, Thewarach .
INFECTION GENETICS AND EVOLUTION, 2014, 21 :443-451
[46]   Genome size estimation of liver fluke Opisthorchis viverrini by real-time polymerase chain reaction based method [J].
Kaewkong, Worasak ;
Imtawil, Kanokwan ;
Maleewong, Wanchai ;
Intapan, Pewpan M. ;
Sri-Aroon, Pusadee ;
Wongkham, Sopit ;
Wongkham, Chaisiri .
PARASITOLOGY INTERNATIONAL, 2012, 61 (01) :77-80
[47]   Genomics and proteomics of the liver fluke Opisthorchis felineus [J].
Mordvinov, V. A. ;
Ershov, N. I. ;
Zaparina, O. G. ;
Pakharukova, M. Y. .
VAVILOVSKII ZHURNAL GENETIKI I SELEKTSII, 2020, 24 (04) :383-390
[48]   Recombinant protein production and functional analysis of a M60-like-2 metallopeptidase enzyme from the carcinogenic liver fluke Opisthorchis viverrini [J].
Jumpajan, Jiraporn ;
Chaiyadet, Sujittra ;
Saichua, Prasert ;
Tangkawatana, Sirikachorn ;
Talabnin, Krajang ;
Laha, Thewarach ;
Suttiprapa, Sutas .
PROTEIN EXPRESSION AND PURIFICATION, 2024, 216
[49]   Carcinogenic liver fluke Opisthorchis viverrini oxysterols detected by LC-MS/MS survey of soluble fraction parasite extract [J].
Vale, Nuno ;
Gouveia, Maria Joao ;
Botelho, Monica ;
Sripa, Banchob ;
Suttiprapa, Sutas ;
Rinaldi, Gabriel ;
Gomes, Paula ;
Brindley, Paul J. ;
Correia da Costa, Jose Manuel .
PARASITOLOGY INTERNATIONAL, 2013, 62 (06) :535-542
[50]   Effects of Bayluscide on Bithynia siamensis goniomphalos, the first intermediate host of the human liver fluke, Opisthorchis viverrini, in laboratory and field trials [J].
Tesana, S. ;
Thapsripair, P. ;
Thammasiri, C. ;
Prasopdee, S. ;
Suwannatrai, A. ;
Harauy, S. ;
Piratae, S. ;
Khampoosa, P. ;
Kulsantiwong, J. ;
Donthaisong, C. ;
Chalokepanrat, P. ;
Viyanant, V. ;
Malone, J. B. .
PARASITOLOGY INTERNATIONAL, 2012, 61 (01) :52-55
12345