Comparative proteomic analysis of juvenile and adult liver fluke, Opisthorchis viverrini

被引：10

作者：

Boonmee, S ^{[1
]}

Imtawil, K ^{[1
]}

Wongkham, C ^{[1
]}

Wongkham, S ^{[1
]}

机构：

[1] Khon Kaen Univ, Fac Med, Dept Biochem, Khon Kaen 40002, Thailand

来源：

ACTA TROPICA | 2003年 / 88卷 / 03期

关键词：

Opisthorchis viverrini; proteomics; 2-DE; liver fluke;

D O I：

10.1016/j.actatropica.2003.09.006

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

We used comparative two-dimensional gel electrophoresis to highlight proteins that are differentially expressed in the maturation stage of the parasite Opisthorchis viverrini (OV). The proteins differentially expressed in the juvenile/adult forms of the parasite are thought to be important for survival and pathogenesis. We used a nonlinear gradient pH ranged 3-10 strips for isoelectric focusing to resolve soluble proteins from four different maturation periods of OV from 1 week juvenile to 4 week adult. Approximately 210-240 protein spots were resolved by 2-DE in two ranges of pI (4.5-5.8 and 6.0-8.0). At least 35 protein spots were differentially expressed in 4 week adult compared to 1 week juvenile fluke. These proteins may involve in sex organ development and egg production. Comparative analysis of the OV proteome of different aged parasites during maturation may help to better understand parasite biology, pathogenesis/carcinogenesis related to this parasite and lead to the identification of new targets of vaccines and drugs. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：233 / 238

页数：6

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