YnfA, a SMR family efflux pump is abundant in Escherichia coli isolates from urinary infection

被引：8

作者：

Sarkar, S. K. ^{[1
]}

Bhattacharyya, A. ^{[1
]}

Mandal, S. M. ^{[2
]}

机构：

[1] Sanjiban Hosp, Dept Pathol & Med, Fuleswar 711316, Howrah, India

[2] Indian Inst Technol Kharagpur, Cent Res Facil, Kharagpur, W Bengal, India

来源：

INDIAN JOURNAL OF MEDICAL MICROBIOLOGY | 2015年 / 33卷 / 01期

关键词：

Antibiotic resistance; efflux pumps; small multi-drug resistance family; ynfA; RESISTANCE; MECHANISM;

D O I：

10.4103/0255-0857.148415

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A quantitative study was undertaken to determine the expression level of different efflux pumps in multi-drug-resistant (MDR) Escherichia coli isolates from urinary infection. We have determined the presence of different efflux pumps and measured the expression level of tolC, mdfA, norE and ynfA genes among 48 isolates by quantitative real-time PCR. The expression level of tolC and ynfA was constantly high and observed among 75-80% of isolates, whereas mdfA and norE were expressed occasionally. Our findings suggest that ynfA, a new SMR efflux pump gene family member increases the antibiotics' resistance in E. coli.

引用

页码：139 / 142

页数：4

共 10 条

[1]

Clinical and Laboratory Standards Institute (CLSI), 2012, PERFORMANCE STANDARD

[2]

Hus Nir, 2005

[3] Virulence Characteristics and Genetic Affinities of Multiple Drug Resistant Uropathogenic Escherichia coli from a Semi Urban Locality in India [J].

Jadhav, Savita ;

Hussain, Arif ;

Devi, Savita ;

Kumar, Ashutosh ;

Parveen, Sana ;

Gandham, Nageshwari ;

Wieler, Lothar H. ;

Ewers, Christa ;

Ahmed, Niyaz .

PLOS ONE, 2011, 6 (03)

[4] Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study [J].

Kumarasamy, Karthikeyan K. ;

Toleman, Mark A. ;

Walsh, Timothy R. ;

Bagaria, Jay ;

Butt, Fafhana ;

Balakrishnan, Ravikumar ;

Chaudhary, Uma ;

Doumith, Michel ;

Giske, Christian G. ;

Irfan, Seema ;

Krishnan, Padma ;

Kumar, Anil V. ;

Maharjan, Sunil ;

Mushtaq, Shazad ;

Noorie, Tabassum ;

Paterson, David L. ;

Pearson, Andrew ;

Perry, Claire ;

Pike, Rachel ;

Rao, Bhargavi ;

Ray, Ujjwayini ;

Sarma, Jayanta B. ;

Sharma, Madhu ;

Sheridan, Elizabeth ;

Thirunarayan, Mandayam A. ;

Turton, Jane ;

Upadhyay, Supriya ;

Warner, Marina ;

Welfare, William ;

Livermore, David M. ;

Woodford, Neil .

LANCET INFECTIOUS DISEASES, 2010, 10 (09) :597-602

[5] Role of Human DNA Glycosylase Nei-like 2 (NEIL2) and Single Strand Break Repair Protein Polynucleotide Kinase 3′-Phosphatase in Maintenance of Mitochondrial Genome [J].

Mandal, Santi M. ;

Hegde, Muralidhar L. ;

Chatterjee, Arpita ;

Hegde, Pavana M. ;

Szczesny, Bartosz ;

Banerjee, Dibyendu ;

Boldogh, Istvan ;

Gao, Rui ;

Falkenberg, Maria ;

Gustafsson, Claes M. ;

Sarkar, Partha S. ;

Hazra, Tapas K. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2012, 287 (04) :2819-2829

[6] OUTER-MEMBRANE BARRIER AS A MECHANISM OF ANTIMICROBIAL RESISTANCE [J].

NIKAIDO, H .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1989, 33 (11) :1831-1836

[7] Clinically relevant chromosomally encoded multidrug resistance efflux pumps in bacteria [J].

Piddock, LJV .

CLINICAL MICROBIOLOGY REVIEWS, 2006, 19 (02) :382-+

[8] Expression of Multidrug Efflux Pump Genes acrAB-tolC, mdfA, and norE in Escherichia coli Clinical Isolates as a Function of Fluoroquinolone and Multidrug Resistance [J].

Swick, Michelle C. ;

Morgan-Linnell, Sonia K. ;

Carlson, Kimberly M. ;

Zechiedrich, Lynn .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2011, 55 (02) :921-924

[9] Mechanisms of antimicrobial resistance in bacteria [J].

Tenover, Fred C. .

AMERICAN JOURNAL OF MEDICINE, 2006, 119 (06) :S3-S10

[10] Multicomponent drug efflux complexes: architecture and mechanism of assembly [J].

Zgurskaya, Helen I. .

FUTURE MICROBIOLOGY, 2009, 4 (07) :919-932

← 1 →