Hyperthermia causes bovine mammary epithelial cell death by a mitochondrial-induced pathway

被引：52

作者：

Du, Juan ^{[1
]}

Di, He-Shuang ^{[1
]}

Gu, Liang ^{[2
]}

Li, Zhong-Hao ^{[1
]}

Wang, Gen-Lin ^{[1
]}

机构：

[1] Naning Agr Univ, Dept Anim Sci & Technol, Nanjing 210095, Peoples R China

[2] Anhui Technol Coll, Dept Anim Sci, Fengyang 233100, Peoples R China

来源：

JOURNAL OF THERMAL BIOLOGY | 2008年 / 33卷 / 01期

关键词：

mammary epithelial cells; apoptosis; necrosis; mitochondria;

D O I：

10.1016/j.jtherbio.2007.06.002

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The effects of mild hyperthermia on bovine mammary epithelial cells exposed to 40 degrees C for 1 h were studied. The results showed that cell viability, ultrastructural features as well as mitochondrial function were significantly influenced by the mild heat treatment (40 degrees C). There was a considerate decrease in cell viability accompanied by cell loss resulting from apoptosis and necrosis followed by G(2)/M arrest. Cell death followed the typical cascade, namely decrease in the ratio of Bcl-2/Bax and mitochondrial membrane potential (Delta Psi(m)), mitochondrial swelling and caspase-3 activities dramatically increased; DNA was also damaged. In conclusion, hyperthermia depresses cell viability and induces bovine mammary cell apoptosis and necrosis through the mitochondrial-triggered cell death pathway. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：37 / 47

页数：11

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