Hyperthermia causes bovine mammary epithelial cell death by a mitochondrial-induced pathway

被引：52

作者：

Du, Juan ^{[1
]}

Di, He-Shuang ^{[1
]}

Gu, Liang ^{[2
]}

Li, Zhong-Hao ^{[1
]}

Wang, Gen-Lin ^{[1
]}

机构：

[1] Naning Agr Univ, Dept Anim Sci & Technol, Nanjing 210095, Peoples R China

[2] Anhui Technol Coll, Dept Anim Sci, Fengyang 233100, Peoples R China

来源：

JOURNAL OF THERMAL BIOLOGY | 2008年 / 33卷 / 01期

关键词：

mammary epithelial cells; apoptosis; necrosis; mitochondria;

D O I：

10.1016/j.jtherbio.2007.06.002

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The effects of mild hyperthermia on bovine mammary epithelial cells exposed to 40 degrees C for 1 h were studied. The results showed that cell viability, ultrastructural features as well as mitochondrial function were significantly influenced by the mild heat treatment (40 degrees C). There was a considerate decrease in cell viability accompanied by cell loss resulting from apoptosis and necrosis followed by G(2)/M arrest. Cell death followed the typical cascade, namely decrease in the ratio of Bcl-2/Bax and mitochondrial membrane potential (Delta Psi(m)), mitochondrial swelling and caspase-3 activities dramatically increased; DNA was also damaged. In conclusion, hyperthermia depresses cell viability and induces bovine mammary cell apoptosis and necrosis through the mitochondrial-triggered cell death pathway. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：37 / 47

页数：11

共 52 条

[11] p53-independent thermosensitization by mitomycin C in human non-small-cell lung cancer cells [J].

Jin, ZH ;

Matsumoto, H ;

Hayashi, S ;

Hatashita, M ;

Ohtsubo, T ;

Shioura, H ;

Kitai, R ;

Kano, E .

INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS, 2004, 59 (03) :852-860

[12] BCL-2 INHIBITION OF NEURAL DEATH - DECREASED GENERATION OF REACTIVE OXYGEN SPECIES [J].

KANE, DJ ;

SARAFIAN, TA ;

ANTON, R ;

HAHN, H ;

GRALLA, EB ;

VALENTINE, JS ;

ORD, T ;

BREDESEN, DE .

SCIENCE, 1993, 262 (5137) :1274-1277

[13] Caspase-independent cell killing by Fas-associated protein with death domain [J].

Kawahara, A ;

Ohsawa, Y ;

Matsumura, H ;

Uchiyama, Y ;

Nagata, S .

JOURNAL OF CELL BIOLOGY, 1998, 143 (05) :1353-1360

[14] Mild heat shock induces autophagic growth arrest, but not apoptosis in U251-MG and U87-MG human malignant glioma cells [J].

Komata, T ;

Kanzawa, T ;

Nashimoto, T ;

Aoki, H ;

Endo, S ;

Nameta, M ;

Takahashi, H ;

Yamamoto, T ;

Kondo, S ;

Tanaka, R .

JOURNAL OF NEURO-ONCOLOGY, 2004, 68 (02) :101-111

[15] Mitochondrial implication in apoptosis. Towards an endosymbiont hypothesis of apoptosis evolution [J].

Kroemer, G .

CELL DEATH AND DIFFERENTIATION, 1997, 4 (06) :443-456

[16] The mitochondrial death/life regulator in apoptosis and necrosis [J].

Kroemer, G ;

Dallaporta, B ;

Resche-Rigon, M .

ANNUAL REVIEW OF PHYSIOLOGY, 1998, 60 :619-642

[17] Heat stress elicits different responses in peripheral blood mononuclear cells from Brown Swiss and Holstein cows [J].

Lacetera, N. ;

Bernabucci, U. ;

Scalia, D. ;

Basirico, L. ;

Morera, P. ;

Nardone, A. .

JOURNAL OF DAIRY SCIENCE, 2006, 89 (12) :4606-4612

[18] MECHANISMS OF MILD HYPERTHERMIA-INDUCED CYTOTOXICITY IN HUMAN PROSTATIC-CARCINOMA CELLS - PERTURBATION OF CELL-CYCLE PROGRESSION AND DNA FRAGMENTATION [J].

LEE, YJ ;

KIM, JH ;

RYU, S ;

KIM, SH ;

VIDAIR, CA ;

ARMOUR, EP ;

CORRY, PM .

JOURNAL OF THERMAL BIOLOGY, 1994, 19 (05) :305-313

[19] The mitochondrial permeability transition in cell death: a common mechanism in necrosis, apoptosis and autophagy [J].

Lemasters, JJ ;

Nieminen, AL ;

Qian, T ;

Trost, LC ;

Elmore, SP ;

Nishimura, Y ;

Crowe, RA ;

Cascio, WE ;

Bradham, CA ;

Brenner, DA ;

Herman, B .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1998, 1366 (1-2) :177-196

[20] Identification of cell types in the developing goat mammary gland [J].

Li, P ;

Wilde, CJ ;

Finch, LMB ;

Fernig, DG ;

Rudland, PS .

HISTOCHEMICAL JOURNAL, 1999, 31 (06) :379-393

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