Endcaps for stabilizing short DNA duplexes

被引：4

作者：

Ng, PS

Pingle, MR

Balasundarum, G

Friedman, A

Zu, XL

Bergstrom, DE

机构：

[1] Purdue Univ, Dept Med Biochem & Mol Pharmacol, W Lafayette, IN 47907 USA

[2] Purdue Univ, Dept Sci Biol, W Lafayette, IN 47907 USA

[3] Walther Canc Inst, Indianapolis, IN USA

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2003年 / 22卷 / 5-8期

关键词：

endcap; amide; terthiophene; stabilization; DNA duplexes;

D O I：

10.1081/NCN-120023088

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The syntheses of endcaps for covalently linking the 3' and 5' hydroxyl groups of blunt end double-stranded DNA are described. Endcap diols were converted into DMTr protected phosphoramidites and incorporated between nucleotides 4 and 5 of a self-complementary octamer. The stabilizing effect of the endcaps on duplex DNA was determined by T-m experiments on the self-complementary octamer.

引用

页码：1635 / 1637

页数：3