Streptomyces ST66 Amylomaltase Gene Cloning and Expression and Production of Cycloamylose

被引：0

作者：

Wang, Shuixing ^{[1
]}

Zhen, Chen ^{[1
]}

Liu, Lianying ^{[2
]}

Wu, Lingwei ^{[1
]}

机构：

[1] Nanchang Univ, Jiangxi OAI Joint Res Inst, Nanchang 330047, Peoples R China

[2] Nanchang Univ, Sch Foreign Language, Nanchang 330047, Peoples R China

来源：

2009 3RD INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICAL ENGINEERING, VOLS 1-11 | 2009年

关键词：

4-alpha-glucanotransferas; cycloamylose; Streptomyces; amylomaltase; ESCHERICHIA-COLI; 4-ALPHA-GLUCANOTRANSFERASE;

D O I：

暂无

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The amylomaltase gene of the Streptomyces ST66 was cloned. The open reading frame of this gene consisted of 2,136 nucleotides and encoded a polypeptide that was711 amino acids. The amylomaltase gene was overexpressed in E. con. The detailed action profile of crude enzyme from E.coli Top 10r/pTrc-CKS-MalQ on amylose was analyzed and tested whether this crude enzyme could catalyze cyclization of amylose. The mixture solution that was treated by crude enzyme resulted in a rapid decrease of absorbance of iodine-amylose complex and the accumulation of a product that was resistant to an exo-amylase (glucoamylase) but was degraded by an endo-type a-amylase to glucose and maltose. These results indicated that the enzyme acted on amylose, it catalyzed an intramolecular transglycosylation (cyclization) reaction which produced cyclic a-1,4-glucan (cycloamylose), like potato D-enzyme.

引用

页码：646 / +

页数：2

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