Streptomyces ST66 Amylomaltase Gene Cloning and Expression and Production of Cycloamylose

被引:0
作者
Wang, Shuixing [1 ]
Zhen, Chen [1 ]
Liu, Lianying [2 ]
Wu, Lingwei [1 ]
机构
[1] Nanchang Univ, Jiangxi OAI Joint Res Inst, Nanchang 330047, Peoples R China
[2] Nanchang Univ, Sch Foreign Language, Nanchang 330047, Peoples R China
来源
2009 3RD INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICAL ENGINEERING, VOLS 1-11 | 2009年
关键词
4-alpha-glucanotransferas; cycloamylose; Streptomyces; amylomaltase; ESCHERICHIA-COLI; 4-ALPHA-GLUCANOTRANSFERASE;
D O I
暂无
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
The amylomaltase gene of the Streptomyces ST66 was cloned. The open reading frame of this gene consisted of 2,136 nucleotides and encoded a polypeptide that was711 amino acids. The amylomaltase gene was overexpressed in E. con. The detailed action profile of crude enzyme from E.coli Top 10r/pTrc-CKS-MalQ on amylose was analyzed and tested whether this crude enzyme could catalyze cyclization of amylose. The mixture solution that was treated by crude enzyme resulted in a rapid decrease of absorbance of iodine-amylose complex and the accumulation of a product that was resistant to an exo-amylase (glucoamylase) but was degraded by an endo-type a-amylase to glucose and maltose. These results indicated that the enzyme acted on amylose, it catalyzed an intramolecular transglycosylation (cyclization) reaction which produced cyclic a-1,4-glucan (cycloamylose), like potato D-enzyme.
引用
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页码:646 / +
页数:2
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