Response of inflammatory markers to balloon angioplasty in peripheral arterial occlusive disease

Objectives: to study the inflammatory response to balloon angioplasty (PTA). Design: prospective study. Materials: blood samples were drawn for cytokine analysis from 10 patients undergoing PTA before, after 60 nrin and 6 h after the balloon inflation. Adhesion molecules were analysed In 14 patients undergoing PTA and in seven patients undergoing angiography only. Arterial samples were taken in eight patients, before PTA, immediately after anti lj min Inter. Venous samples were taken in six patients and ill the group undergoing angiography only. The sampling was before, 60, 90 and 120 min after the procedure. As controls served 15 patients with no signs of peripheral arterial disease. Methods: cytokines (IL-6, TNF-alpha) were analysed using ELISA. Adhesion molecule expression on WBC was measured by flow cytometry. Results: a significant increase of IL-6 in the sample taken 6h after the last balloon inflation was seen in five patients. TNF-cr items raised only in one patient. The group of patients with peripheral arterial occlusive disease (PAOD) expressed pre-interventionally a higher level of adhesion molecules on WBC compared to the controls. The expression of adhesion molecules (CD11b/CD18) was significantly decreased after PTA. Conclusions: only a very limited cytokine response is caused by PTA reflecting the small surgical trauma. PTA results in a downregulation of detectable CD11b/CD18 expression on WBC in the circulation, which may reflect removal of activated cells through adhesion and extravasation.