Cryopreservation of Human Adipose Tissues and Adipose-Derived Stem Cells with DMSO and/or Trehalose: A Systematic Review

被引:23
作者
Crowley, Conor A. [1 ]
Smith, William P. W. [2 ]
Seah, K. T. Matthew [3 ]
Lim, Soo-Keat [4 ]
Khan, Wasim S. [3 ]
机构
[1] Australasian Coll Cosmet Surg, Parramatta, NSW 2150, Australia
[2] Univ Cambridge, Sch Clin Med, Cambridge CB2 0SP, England
[3] Univ Cambridge, Addenbrookes Hosp, Div Trauma & Orthopaed Surg, Cambridge CB2 0QQ, England
[4] Ashbrook Cosmet Surg, Mosman, NSW 2088, Australia
关键词
adipose-derived stem cells; cryopreservation; trehalose; lipoaspirate; autologous fat grafting; ageing; VIABILITY; SEARCH;
D O I
10.3390/cells10071837
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Adipose tissue senescence is implicated as a major player in obesity- and ageing-related disorders. There is a growing body of research studying relevant mechanisms in age-related diseases, as well as the use of adipose-derived stem cells in regenerative medicine. The cell banking of tissue by utilising cryopreservation would allow for much greater flexibility of use. Dimethyl sulfoxide (DMSO) is the most commonly used cryopreservative agent but is toxic to cells. Trehalose is a sugar synthesised by lower organisms to withstand extreme cold and drought that has been trialled as a cryopreservative agent. To examine the efficacy of trehalose in the cryopreservation of human adipose tissue, we conducted a systematic review of studies that used trehalose for the cryopreservation of human adipose tissues and adipose-derived stem cells. Thirteen articles, including fourteen studies, were included in the final review. All seven studies that examined DMSO and trehalose showed that they could be combined effectively to cryopreserve adipocytes. Although studies that compared nonpermeable trehalose with DMSO found trehalose to be inferior, studies that devised methods to deliver nonpermeable trehalose into the cell found it comparable to DMSO. Trehalose is only comparable to DMSO when methods are devised to introduce it into the cell. There is some evidence to support using trehalose instead of using no cryopreservative agent.
引用
收藏
页数:14
相关论文
共 50 条
[41]   The effects of human keratinocyte coculture on human adipose-derived stem cells [J].
Seo, Bommie F. ;
Kim, Ki J. ;
Kim, Min K. ;
Rhie, Jong W. .
INTERNATIONAL WOUND JOURNAL, 2016, 13 (05) :630-635
[42]   Adipose-Derived Tissue in the Treatment of Dermal Fibrosis: Antifibrotic Effects of Adipose-Derived Stem Cells [J].
Borovikova, Anna A. ;
Ziegler, Mary E. ;
Banyard, Derek A. ;
Wirth, Garrett A. ;
Paydar, Keyianoosh Z. ;
Evans, Gregory R. D. ;
Widgerow, Alan David .
ANNALS OF PLASTIC SURGERY, 2018, 80 (03) :297-307
[43]   Combining decellularized human adipose tissue extracellular matrix and adipose-derived stem cells for adipose tissue engineering [J].
Wang, Lina ;
Johnson, Joshua A. ;
Zhang, Qixu ;
Beahm, Elisabeth K. .
ACTA BIOMATERIALIA, 2013, 9 (11) :8921-8931
[44]   Evaluation of the viability and osteogenic differentiation of cryopreserved human adipose-derived stem cells [J].
Liu, Guangpeng ;
Zhou, Heng ;
Li, Yulin ;
Li, Gang ;
Cui, Lei ;
Liu, Wei ;
Cao, Yilin .
CRYOBIOLOGY, 2008, 57 (01) :18-24
[45]   Adipose-derived stem cells from the breast [J].
Yang, Jie ;
Xiong, Lingyun ;
Wang, Rongrong ;
Sun, Jiaming ;
Hirche, Christoph .
JOURNAL OF RESEARCH IN MEDICAL SCIENCES, 2014, 19 (02) :112-116
[46]   Adipose-derived stem cells for wound healing [J].
Hassanshahi, Alireza ;
Hassanshahi, Mohammadhossein ;
Khabbazi, Samira ;
Hosseini-Khah, Zahra ;
Peymanfar, Yaser ;
Ghalamkari, Saman ;
Su, Yu-Wen ;
Xian, Cory J. .
JOURNAL OF CELLULAR PHYSIOLOGY, 2019, 234 (06) :7903-7914
[47]   Neurogenesis of Adipose-derived Stem Cells in Hydrogel [J].
谢雪微 ;
唐洲平 ;
陈娟 ;
杨洁 ;
曾文高 ;
刘娜 ;
刘永明 .
Current Medical Science, 2011, 31 (02) :174-177
[48]   Neurogenesis of Adipose-derived Stem Cells in Hydrogel [J].
Xie, Xuewei ;
Tang, Zhouping ;
Chen, Juan ;
Yang, Jie ;
Zeng, Wengao ;
Liu, Na ;
Liu, Yongming .
JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES, 2011, 31 (02) :174-177
[49]   Hormesis and adult adipose-derived stem cells [J].
Calabrese, Edward J. .
PHARMACOLOGICAL RESEARCH, 2021, 172
[50]   Adipose-derived stem cells in orthopaedic pathologies [J].
Usuelli, Federico Giuseppe ;
D'Ambrosi, Riccardo ;
Maccario, Camilla ;
Indino, Cristian ;
Manzi, Luigi ;
Maffulli, Nicola .
BRITISH MEDICAL BULLETIN, 2017, 124 (01) :31-54