Validation of a Multiplex Real-Time PCR Assay for Detection of Mycobacterium spp., Mycobacterium tuberculosis Complex, and Mycobacterium avium Complex Directly from Clinical Samples by Use of the BD Max Open System

被引：18

作者：

Rocchetti, Talita T. ^{[1
,2
]}

Silbert, Suzane ^{[1
]}

Gostnell, Alicia ^{[1
]}

Kubasek, Carly ^{[1
]}

Widen, Raymond ^{[1
]}

机构：

[1] Tampa Gen Hosp, Dept Pathol, Esoter Testing Lab, Tampa, FL 33606 USA

[2] Univ Fed Sao Paulo, UNIFESP, LEMC, Sao Paulo, Brazil

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2016年 / 54卷 / 06期

关键词：

RAPID DIAGNOSIS; HERPES-SIMPLEX; DNA; DIFFERENTIATION; PERFORMANCE; INHIBITORS; SPUTUM;

D O I：

10.1128/JCM.00241-16

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A multiplex real-time PCR was validated on the BD Max open system to detect different Mycobacterium tuberculosis complex, Mycobacterium avium complex, and Mycobacterium spp. directly from clinical samples. The PCR results were compared to those with traditional cultures. The multiplex PCR assay was found to be a specific and sensitive method for the rapid detection of mycobacteria directly from clinical specimens.

引用

页码：1644 / 1647

页数：4