MESENCHYMAL STEM CELLS IMMUNE PROPERTIES AS SENSORS OF MICROENVIRONMENT

Tissue inflammation leads to the recruitment and activation of several cell types, which cooperate to create a particular microenvironment that determines the regression of the pathology or its perpetuation (chronic inflammation). Mesenchymal stem cells (MSCs) are multipotent cells present in the stromal fraction of many tissues (i.e umbilical cord blood, placenta, adipose tissue). Several studies are now showing MSCs potential in tissue engineering, especially because of their ability to differentiate onto nanostructure surface. However the effect of nanostructure on MSCs immune properties is not properly characterized. The aim of the project is to develop MSCs reporter lines for transcription factors associated to inflammation, in order to monitor their activity once the cells are seeded onto nanostructure. This will be helpful to understand, which materials are more suitable in term of tissue regeneration.