Antibacterial and antioxidant activities of compounds isolated from the leaves of Symphonia globulifera (Clusiaceae) and their chemophenetic significance

The phytochemical study was done on the methanol extract from of the leaves of Symphonia globulifera. This plant has been used in traditional medicine to treat of different ailments such as malaria, diseases of the skin, diabetes, cough, intestinal worms, pre-hepatic jaundice and fever. Chromatographic fractionation and purification of this extract led to the isolation and characterization of twelve compounds (1-12) including pristriol (1), robusta-flavone (2), polycarpol (3), 7"-O-methylrobustaflavone (4), amentoflavone (5), stigmasterol glucoside (6), epicatechin (7), apigenin (8), luteolin (9), 1,5-dihydroxyxanthone (10), beta-sitosterol 3-beta-D-glucopyranoside (11) and a mixture of stigmasterol and beta-sitosterol (12). The structures of compounds 1-12 were elucidated on the basis of 1D and 2D NMR, mass spectrometric and the spectroscopic data as well as comparison with the liter-ature. All these compounds were isolated for the first time from Symphonia genus. The NMR spectra and structure elucidation of compound 1 were reported for the first in the literature. The antibacterial and antioxidant ac-tivities of three of these compounds were evaluated. The chemophenetic significance of these compounds is also discussed.