Modified multiplex PCR for identification of Bacillus Calmette-Guerin substrain Tokyo among clinical isolates

被引：5

作者：

Seki, M

Sato, A

Honda, I

Yamazaki, T

Yano, I

Koyama, A

Toida, I

机构：

[1] Japan BCG Lab, Kiyose, Tokyo 2040022, Japan

[2] Natl Inst Infect Dis, Dept Bacteriol, Shinjuku Ku, Tokyo 1620052, Japan

来源：

VACCINE | 2005年 / 23卷 / 24期

关键词：

identify BCG; adverse reaction; multiplex PCR;

D O I：

10.1016/j.vaccine.2004.12.016

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

When an adverse reaction occurs and a mycobacterial species is isolated from a person vaccinated with Bacillus Calmette-Guerin (BCG) or a patient receiving BCG immunotherapy, it is essential to identify whether the isolate is BCG or another mycobacterial species. However, differentiation of BCG from other members of Mycobacterium tuberculosis complex has been very difficult. Using several specific primer-pairs, Bedwell et al. [Bedwell J, Kairo SK, Behr MA, Bygraves JA. Identification of substrains of BCG vaccine using multiplex PCR. Vaccine 2001; 19: 2146-51] recently reported that they could distinguish BCG substrains. We modified their method to improve differentiation of Tokyo 172 from other members of the M. tuberculosis complex, and examined whether this modified method could be applied to clinical isolates. Our method clearly identified BCG substrain (BCG Tokyo 172) among clinical isolates and easily distinguished between M. tuberculosis and wild-type Mycobacterium bovis. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：3099 / 3102

页数：4

共 4 条

[1] Identification of substrains of BCG vaccine using multiplex PCR [J].