A new multiplex PCR strategy for the simultaneous determination of four genetic polymorphisms affecting HIV-1 disease progression

被引：36

作者：

Kristiansen, TB

Knudsen, TB

Ohlendorff, S

Eugen-Olsen, J

机构：

[1] Univ Copenhagen, Hvidovre Hosp, Clin Res Unit 136, DK-2650 Copenhagen, Denmark

[2] Univ Copenhagen, Hvidovre Hosp, Dept Infect Dis, Copenhagen, Denmark

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2001年 / 252卷 / 1-2期

关键词：

chemokine receptors; HIV; CCR5; Delta; 32; CCR2; 64I; SDF1 3 ' A; CCR5 promoter 59029 A/G transition;

D O I：

10.1016/S0022-1759(01)00349-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The CCR5 Delta 32, CCR2 64I, SDF1 3'A, and CCR5 promoter 59029 polymorphisms have been suggested to influence HIV-I disease progression. Furthermore, the CCR5 Delta 32 and the CCR2 64I polymorphisms have been associated with various other diseases, The purpose of the present study was to develop a multiplex assay for the simultaneous determination of these four polymorphisms. Results obtained with the multiplex assay were compared to results obtained by conventional RFLP-PCR and no differences were observed. The multiples assay offers a quick tool for the determination of the CCR5 Delta 32, CCR2 64I, SDF1 3'A, and CCR5 promoter 59029 A/G polymorphisms. (C) 2001 Elsevier Science B.V. All rights reserved.

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页码：147 / 151

页数：5

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