Preparation of the honeycomb patterned porous film of biodegradable polymer for tissue engineering scaffolds

Fabrication and surface modification of a cell scaffold are very important in tissue engineering. In this study, the effects of pore size and surface wettability on hepatocyte adhesion and morphology was studied, using the porous poly-epsilon-caprolactone (PCL) film prepared by a simple casting technique. The degree of spreading of hepatocyte was enhanced with the decrease of the pore size (similar to 10 mu m). Hepatocyte motility and spreading were restricted on the patterned film having cellular-sized pores (ca. 20 mu m). Partial hydrolysis of the film surface enhanced the migration and spheroid formation of the cultured hepatocyte.